PXD040923
PXD040923 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | 1/4 MIC Contezolid, 1/4 MIC Linezolid and DMSO control group of Staphylococcus aureus biofilm quantitative proteomics comparative study to observe the global expression of protein Contezolid, Linezolid treatment groups and DMSO control group |
| Description | S. aureus isolate in stable phase were inoculated into 15 ml TSBG plate culture, which contained 1/4 MIC of contezolid and linezolid treatment (DMSO as control) with 3 technical replicates in each group,. Incubate at 37 ° for 16 h and centrifuge at 5,000 rpm for 5 min to collect bacteria, including the formed biofilm bacteria. Wash with 1 ml PBS in water twice, transferred to a 1.5 ml EP tube, centrifuge and discard the supernatant. Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer. |
| HostingRepository | iProX |
| AnnounceDate | 2023-03-16 |
| AnnouncementXML | Submission_2023-03-16_19:50:33.320.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xinyi Cao |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-03-16 19:08:50 | ID requested | |
| 1 | 2023-03-16 19:08:58 | announced | |
| ⏵ 2 | 2023-03-16 19:50:33 | announced | 2023-03-17: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Staphylococcus aureus, contezolid, linezolid |
Contact List
| Zewen Wen | |
|---|---|
| contact affiliation | Nanshan Hospital |
| contact email | wenzw05@163.com |
| lab head | |
| Xinyi Cao | |
| contact affiliation | Nanshan Hospital |
| contact email | caoxinyi0120@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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