PXD040916

PXD040916 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome analysis of Mel426 melanoma cells with R2PD1 treatment
Description	A label-free quantification experiment was performed to study the effect of R2PD1 chimeric protein treatment on the cellular proteome of Mel426 human melanoma cells. Peptides were separated by online reverse phase liquid chromatography and measured in an Exploris 480 Orbitrap mass spectrometer. Mass spectrometry raw data were processed by MaxQuant software package (version 2.1.3.0) using its built-in Andromeda search engine. Mass spectra were searched against a target-decoy database consisting of the forward and reverse sequences of UniProt human reference proteome (release 2022_04; 102,601 entries) and a list of 246 common contaminants. Trypsin/P specificity was chosen. The minimum peptide length was set to be 7 amino acids. False discovery rate (FDR) was set to 1% for both peptide and protein identifications. MaxLFQ algorithm was employed for protein quantification.
HostingRepository	MassIVE
AnnounceDate	2023-05-24
AnnouncementXML	Submission_2023-05-24_05:55:36.115.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	J Chen
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	iodoacetamide - site C
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-16 08:11:38	ID requested
⏵ 1	2023-05-24 05:55:36	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: proteome, Mel426, melanoma, R2PD1

submitter keyword: proteome, Mel426, melanoma, R2PD1

Contact List

Christof Niehrs
contact affiliation	Institute of Molecular Biology
contact email	C.Niehrs@imb-mainz.de
lab head
J Chen
contact affiliation	Institute of Molecular Biology
contact email	j.chen@imb-mainz.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091500/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091500/