PXD040906
PXD040906 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep |
Description | The study was conducted to characterize the protein profiles at four key stages during postnatal testicular development, including infant (0-month-old, M0), puberty (3-month-old, M3), sexual maturity (6-month-old, M6) and body maturity (12-month-old, M12), and between the large- and small-testis groups at 6 months in Hu sheep. Consequently, 5252 proteins were identified using isobaric tags for relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods, and 465, 1261, 231 and 1080 differentially abundant proteins (DAPs) were found between M0_vs_M3, M3_vs_M6L, M6L_vs_M12, and M6L_vs_M6S, respectively. GO and KEGG analysis revealed that the majority of DAPs were involved in cellular process, metabolic process and immune system-related pathways. Furthermore, a protein-protein interaction (PPI) network was constructed using the 86 fertility-related DAPs, and five proteins with the highest degree were represented as hub proteins, including CTNNB1, ADAM2, ACR, HSPA2 and GRB2. This study provided new insights into the regulation mechanisms of postnatal testicular development and identified several potential biomarkers for selecting the high-fertility rams. |
HostingRepository | iProX |
AnnounceDate | 2023-07-26 |
AnnouncementXML | Submission_2024-05-27_21:21:53.719.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shengwei Pei |
SpeciesList | scientific name: Ovis sp. LF-2011; NCBI TaxID: 1110706; |
ModificationList | iTRAQ8plex reporter+balance reagent acylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-03-15 20:30:26 | ID requested | |
1 | 2023-07-25 19:37:29 | announced | |
⏵ 2 | 2024-05-27 21:21:54 | announced | 2024-05-28: Update information. |
Publication List
Pei S, Luo J, Weng X, Xu Y, Bai J, Li F, Li W, Yue X, iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep. J Proteomics, 286():104956(2023) [pubmed] |
Keyword List
submitter keyword: sheep, proteomics, testicular development, fertility |
Contact List
Xiangpeng Yue | |
---|---|
contact affiliation | Lanzhou University |
contact email | lexp@lzu.edu.cn |
lab head | |
Shengwei Pei | |
contact affiliation | Lanzhou University |
contact email | peishw2021@lzu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |