PXD040906

PXD040906 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep
Description	The study was conducted to characterize the protein profiles at four key stages during postnatal testicular development, including infant (0-month-old, M0), puberty (3-month-old, M3), sexual maturity (6-month-old, M6) and body maturity (12-month-old, M12), and between the large- and small-testis groups at 6 months in Hu sheep. Consequently, 5252 proteins were identified using isobaric tags for relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods, and 465, 1261, 231 and 1080 differentially abundant proteins (DAPs) were found between M0_vs_M3, M3_vs_M6L, M6L_vs_M12, and M6L_vs_M6S, respectively. GO and KEGG analysis revealed that the majority of DAPs were involved in cellular process, metabolic process and immune system-related pathways. Furthermore, a protein-protein interaction (PPI) network was constructed using the 86 fertility-related DAPs, and five proteins with the highest degree were represented as hub proteins, including CTNNB1, ADAM2, ACR, HSPA2 and GRB2. This study provided new insights into the regulation mechanisms of postnatal testicular development and identified several potential biomarkers for selecting the high-fertility rams.
HostingRepository	iProX
AnnounceDate	2023-07-26
AnnouncementXML	Submission_2024-05-27_21:21:53.719.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shengwei Pei
SpeciesList	scientific name: Ovis sp. LF-2011; NCBI TaxID: 1110706;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 20:30:26	ID requested
1	2023-07-25 19:37:29	announced
⏵ 2	2024-05-27 21:21:54	announced	2024-05-28: Update information.

Pei S, Luo J, Weng X, Xu Y, Bai J, Li F, Li W, Yue X, iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep. J Proteomics, 286():104956(2023) [pubmed]

submitter keyword: sheep, proteomics, testicular development, fertility

Xiangpeng Yue
contact affiliation	Lanzhou University
contact email	lexp@lzu.edu.cn
lab head
Shengwei Pei
contact affiliation	Lanzhou University
contact email	peishw2021@lzu.edu.cn
dataset submitter

iProX dataset URI