PXD040899

PXD040899 is an original dataset announced via ProteomeXchange.

Title	The mechanism to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes
Description	Endoplasmic reticulum (ER)-associated degradation (ERAD) and ER-phagy are two principal degradative mechanisms in the ER; however, the crosstalk between these two pathways and its physiological significance remain unexplored. Here we report that SEL1L-HRD1 ERAD limits autophagy and that, when ERAD is impaired, the ER becomes fragmented containing misfolded ER proteins or aggregates, which are subsequently cleared by autophagy. When both are compromised, ER fragments containing misfolded proteins spatially coalesce into a distinct architecture termed Coalescence of ER Fragments (CERFs), consisted of lipoprotein lipase (LPL) and ER chaperones such as BiP. CERFs enlarge and become increasingly insoluble with age. Finally, we reconstitute the CERFs through LPL and BiP phase separation in vitro, a process controlled by both redox environment and C-terminal tryptophan loop of LPL. Hence, our findings demonstrate a profound cellular adaptation capability and plasticity, centered around SEL1L-HRD1 ERAD, in dealing with misfolded proteins or protein aggregates in the ER.
HostingRepository	PRIDE
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-03_05:48:53.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoqiong Wei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 12:56:43	ID requested
1	2023-04-25 11:19:38	announced
⏵ 2	2024-10-03 05:48:54	announced	2024-10-03: Updated project metadata.

Wu SA, Shen C, Wei X, Zhang X, Wang S, Chen X, Torres M, Lu Y, Lin LL, Wang HH, Hunter AH, Fang D, Sun S, Ivanova MI, Lin Y, Qi L, The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes. Nat Commun, 14(1):3132(2023) [pubmed]

10.1038/s41467-023-38690-4;

submitter keyword: autophagy, lipoprotein lipase, tryptophan loop,SEL1L-HRD1 ERAD, CERFs, BiP, ER-phagy, protein aggregation, phase separation, soluble-to-insoluble phase transition

Ling Qi
contact affiliation	The University of Michigan Medical School
contact email	lingq@med.umich.edu
lab head
Xiaoqiong Wei
contact affiliation	University of Michigan
contact email	weixi@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040899

PRIDE project URI

• PRIDE
  1. PXD040899
     1. Label: PRIDE project
     2. Name: The mechanism to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes