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PXD040895

PXD040895 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling - Participant Group 1
DescriptionBacterial vaginosis (BV) is characterized by depletion of Lactobacillus and overgrowth of anaerobic and facultative bacteria, leading to increased mucosal inflammation, epithelial disruption, and poor reproductive health outcomes. However, the molecular mediators contributing to vaginal epithelial dysfunction are poorly understood. Here we utilized proteomic, transcriptomic and metabolomic analyses to characterize biological features underlying BV in 405 African women and explored functional mechanisms using bacterial co-culturesin vitro. We identified five major vaginal microbiome groups, (L.crispatus(21%), L.iners(18%), any non-specific Lactobacillus species(9%), Gardnerella species .vaginalis(30%), or polymicrobial(22%)). Using multi-‘omics we show that BV associated epithelial disruption and mucosal inflammation are linked to the mammalian target of rapamycin (mTOR) pathway and associate with Gardnerella.vaginalis, M.mulieris, and specific metabolites including imidazole propionate. Bacterial co-culturesExperiments in vitro confirmed that type strain G.vaginalis and, M.mulieris supernatants and, as well as, and imidazole propionate, directly affect epithelial barrier function and , accompanied by activation of mTOR pathways. These results establish the microbiome-mTOR axis as a central feature of epithelial dysfunction in BV.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:04:04.841.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKenzie Birse
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-15 11:32:16ID requested
12023-07-20 15:11:51announced
22023-11-14 09:04:11announced2023-11-14: Updated project metadata.
Publication List
Berard AR, Brubaker DK, Birse K, Lamont A, Mackelprang RD, No, ë, l-Romas L, Perner M, Hou X, Irungu E, Mugo N, Knodel S, Muwonge TR, Katabira E, Hughes SM, Levy C, Calienes FL, Lauffenburger DA, Baeten JM, Celum C, Hladik F, Lingappa J, Burgener AD, Vaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling. Cell Rep, 42(5):112474(2023) [pubmed]
Keyword List
submitter keyword: Human, LC-MSMS, Bacterial Vaginosis, Microbiome, Proteome
Contact List
Adam Burgener
contact affiliationProfessor, Department of Pathology, School of Medicine Director, Center for Global Health and Diseases, School of Medicine, Case Western Reserve University 10900 Euclid Avenue, Cleveland, Ohio, 44106
contact emailadam.burgener@case.edu
lab head
Kenzie Birse
contact affiliationUniversity of Manitoba
contact emailkenzie.birse@umanitoba.ca
dataset submitter
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Dataset FTP location
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