PXD040888

PXD040888 is an original dataset announced via ProteomeXchange.

Title	RNA polymerase II acts to promote the organization of chromatin following DNA replication
Description	In mammals, following the passage of the replisome, nucleosomes lose their defined positioning and transcription contributes to their re-organisation. However, whether transcription plays a greater role in the organization of chromatin following DNA replication remains unclear. The data uploaded are associated to the iPOND-TMT data uploaded under PXD046514 where protein re-association to newly replicated DNA was monitored upon transcription inhibition. To see whether changes observed in the iPOND-TMT time course experiments (PXD046514) are due to changes in overall protein abundance, whole extract samples were taken for each time point (Nascent, 1h and 2h after EdU labelling) and analysed by TMT-Mass Spec.
HostingRepository	PRIDE
AnnounceDate	2024-02-14
AnnouncementXML	Submission_2024-02-14_07:36:35.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susanne Bandau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 04:31:43	ID requested
⏵ 1	2024-02-14 07:36:36	announced

10.1038/s44319-024-00085-x;

Bandau S, Alvarez V, Jiang H, Graff S, Sundaramoorthy R, Gierlinski M, Toman M, Owen-Hughes T, Sidoli S, Lamond A, Alabert C, RNA polymerase II promotes the organization of chromatin following DNA replication. EMBO Rep, 25(3):1387-1414(2024) [pubmed]

submitter keyword: TMT,Proteomics, DNA replications

Constance Alabert
contact affiliation	Centre for Gene Regulation and Expression, School of Life Science, University of Dundee
contact email	c.alabert@dundee.ac.uk
lab head
Susanne Bandau
contact affiliation	University of Dundee
contact email	sbandau@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040888

PRIDE project URI

• PRIDE
  1. PXD040888
     1. Label: PRIDE project
     2. Name: RNA polymerase II acts to promote the organization of chromatin following DNA replication