PXD040883

PXD040883 is an original dataset announced via ProteomeXchange.

Title	Profiling of Serum N-glycosylation in Non-small-cell Lung Cancer
Description	Aberrant serum N-glycan profiles have been observed in multiple cancers including non-small-cell lung cancer (NSCLC), yet the potential of N-glycans in the early diagnosis of NSCLC remains to be determined. Here, serum N-glycan profiles of 275 NSCLC patients and 309 healthy controls were characterized by MALDI-TOF-MS. The levels of serum N-glycans and N-glycosylation patterns were compared between NSCLC and control groups. In addition, a panel of N-glycan biomarkers for NSCLC diagnosis was established and validated using machine learning algorithms. As a result, a total of 54 N-glycan structures were identified in human serum. Compared with healthy controls, 29 serum N-glycans were up- or down-regulated in NSCLC patients. N-glycan abundance in different histological types or clinical stages of NSCLC presented differentiated changes. Furthermore, an optimal biomarker panel of 8 N-glycans was constructed based on logistic regression, with an AUC of 0.86 in the validation set. Notably, this model also showed a desirable capacity in distinguishing early-stage patients from healthy controls (AUC = 0.88). In conclusion, our work highlights the abnormal N-glycan profiles in NSCLC and provides supports for the promising potential of N-glycan panels in clinical NSCLC detection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:15:42.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liming Cheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 00:22:31	ID requested
1	2023-11-23 06:29:32	announced
⏵ 2	2024-10-22 06:15:43	announced	2024-10-22: Updated project metadata.

Wang Y, Liu S, Li J, Yin T, Liu Y, Wang Q, Liu X, Cheng L, Comprehensive serum N-glycan profiling identifies a biomarker panel for early diagnosis of non-small-cell lung cancer. Proteomics, 23(20):e2300140(2023) [pubmed]

10.1002/pmic.202300140;

submitter keyword: Human, MALDI-TOF-MS, Serum, Lung

Liming Cheng
contact affiliation	Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	chengliming2015@163.com
lab head
Liming Cheng
contact affiliation	Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	chengliming2015@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD040883

PRIDE project URI

• PRIDE
  1. PXD040883
     1. Label: PRIDE project
     2. Name: Profiling of Serum N-glycosylation in Non-small-cell Lung Cancer