PXD040880

PXD040880 is an original dataset announced via ProteomeXchange.

Title	A Simplified and Ultrafast Pipeline for Site-specific Quantitative Chemical Proteomics
Description	Activity-based proteome profiling (ABPP) is a powerful chemoproteomic technology for global profiling of protein reactivity and modifications. The tandem orthogonal proteolysis-ABPP (TOP-ABPP) strategy utilizes a clickable enrichment tag with cleavable linkers to enable direct identification of probe-labeled residue sites within the target proteins. However, such a site-specific chemoproteomic workflow requires long operation time and complex sample preparation procedures, limiting its wide applications. In the current study, we developed a Simplified and Ultrafast Peptide Enrichment and Release TOP-ABPP (superTOP-ABPP) pipeline for site-specific quantitative chemoproteomic analysis with special agarose resins that are functionalized with azide groups and acid-cleavable linkers. The azide groups allow enrichment of the alkynyl probe labeled peptides through one-step click reaction, which can be conveniently released by acid cleavage for subsequent LC-MS/MS analysis. In comparison with the traditional TOP-ABPP method, superTOP-ABPP cuts down the averaged sample preparation time from 25 to 9 h, and significantly improves the sensitivity and coverage of site-specific cysteinome profiling. The method can also be seamlessly integrated with reductive dimethylation to enable quantitative chemoproteomic analysis with high accuracy. The simplified and ultrafast superTOP-ABPP will become a valuable tool for site-specific quantitative chemoproteomic studies.
HostingRepository	iProX
AnnounceDate	2023-03-15
AnnouncementXML	Submission_2024-06-06_00:58:01.153.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weidi Xiao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-14 20:43:08	ID requested
1	2023-12-03 18:57:18	announced
⏵ 2	2024-06-06 00:58:01	announced	2024-06-06: Update information.

Xiao W, Chen Y, Zhang J, Guo Z, Hu Y, Yang F, Wang C, A Simplified and Ultrafast Pipeline for Site-Specific Quantitative Chemical Proteomics. J Proteome Res, 22(10):3360-3367(2023) [pubmed]

submitter keyword: ABPP, chemoproteomics, cysteinome, quantitative profiling, peptide enrichment

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Weidi Xiao
contact affiliation	Peking university
contact email	weidixiao@126.com
dataset submitter

iProX dataset URI