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PXD040877

PXD040877 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of the O-Glycoproteome of Flavobacterium johnsoniae
DescriptionFlavobacterium johnsoniae is a free-living member of the Bacteroidota phylum found in soil and water. It is frequently used as a model species for studying a type of gliding motility dependent on the type IX secretion system (T9SS). O-glycosylation has been reported in several Bacteroidota species and the O-glycosylation of S-layer proteins in Tannerella forsythia was shown to be important for certain virulence features. In this study we characterised the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins. The structure of the major glycan was found to be a hexasaccharide with the sequence Hex–(Me-dHex)–Me-HexA–Pent–HexA–Me-HexNAcA. Bioinformatic localisation of the glycoproteins determined 68 inner membrane proteins, 60 periplasmic proteins, 26 outer membrane proteins, 57 lipoproteins and 9 proteins secreted by the T9SS. The glycosylated sites were predominantly located in the periplasm where they are postulated to be beneficial for protein folding/stability. Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated.
HostingRepositoryPRIDE
AnnounceDate2023-04-18
AnnouncementXMLSubmission_2023-04-18_15:43:00.552.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD040877
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPaulVeith
SpeciesList scientific name: Flavobacterium johnsoniae UW101; NCBI TaxID: 376686;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-14 19:36:59ID requested
12023-04-18 15:43:00announced
Publication List
10.6019/PXD040877;
Keyword List
submitter keyword: Flavobacterium johnsoniae, Glycoproteins, Glycans, O-glycosylation, Proteome, Mass spectrometry
Contact List
Eric CReynolds
contact affiliationMelbourne Dental School, The University of Melbourne
contact emaile.reynolds@unimelb.edu.au
lab head
PaulVeith
contact affiliationMelbourne Dental School
contact emailpdv@unimelb.edu.au
dataset submitter
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Dataset FTP location
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