PXD040877 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of the O-Glycoproteome of Flavobacterium johnsoniae |
Description | Flavobacterium johnsoniae is a free-living member of the Bacteroidota phylum found in soil and water. It is frequently used as a model species for studying a type of gliding motility dependent on the type IX secretion system (T9SS). O-glycosylation has been reported in several Bacteroidota species and the O-glycosylation of S-layer proteins in Tannerella forsythia was shown to be important for certain virulence features. In this study we characterised the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins. The structure of the major glycan was found to be a hexasaccharide with the sequence Hex–(Me-dHex)–Me-HexA–Pent–HexA–Me-HexNAcA. Bioinformatic localisation of the glycoproteins determined 68 inner membrane proteins, 60 periplasmic proteins, 26 outer membrane proteins, 57 lipoproteins and 9 proteins secreted by the T9SS. The glycosylated sites were predominantly located in the periplasm where they are postulated to be beneficial for protein folding/stability. Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-18 |
AnnouncementXML | Submission_2023-04-18_15:43:00.552.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD040877 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | PaulVeith |
SpeciesList | scientific name: Flavobacterium johnsoniae UW101; NCBI TaxID: 376686; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-14 19:36:59 | ID requested | |
⏵ 1 | 2023-04-18 15:43:00 | announced | |
Publication List
Keyword List
submitter keyword: Flavobacterium johnsoniae, Glycoproteins, Glycans, O-glycosylation, Proteome, Mass spectrometry |
Contact List
Eric CReynolds |
contact affiliation | Melbourne Dental School, The University of Melbourne |
contact email | e.reynolds@unimelb.edu.au |
lab head | |
PaulVeith |
contact affiliation | Melbourne Dental School |
contact email | pdv@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040877
- Label: PRIDE project
- Name: Characterization of the O-Glycoproteome of Flavobacterium johnsoniae