PXD040877

PXD040877 is an original dataset announced via ProteomeXchange.

Title	Characterization of the O-Glycoproteome of Flavobacterium johnsoniae
Description	Flavobacterium johnsoniae is a free-living member of the Bacteroidota phylum found in soil and water. It is frequently used as a model species for studying a type of gliding motility dependent on the type IX secretion system (T9SS). O-glycosylation has been reported in several Bacteroidota species and the O-glycosylation of S-layer proteins in Tannerella forsythia was shown to be important for certain virulence features. In this study we characterised the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins. The structure of the major glycan was found to be a hexasaccharide with the sequence Hex–(Me-dHex)–Me-HexA–Pent–HexA–Me-HexNAcA. Bioinformatic localisation of the glycoproteins determined 68 inner membrane proteins, 60 periplasmic proteins, 26 outer membrane proteins, 57 lipoproteins and 9 proteins secreted by the T9SS. The glycosylated sites were predominantly located in the periplasm where they are postulated to be beneficial for protein folding/stability. Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated.
HostingRepository	PRIDE
AnnounceDate	2023-04-18
AnnouncementXML	Submission_2023-04-18_15:43:00.552.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040877
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	PaulVeith
SpeciesList	scientific name: Flavobacterium johnsoniae UW101; NCBI TaxID: 376686;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-14 19:36:59	ID requested
⏵ 1	2023-04-18 15:43:00	announced

10.6019/PXD040877;

submitter keyword: Flavobacterium johnsoniae, Glycoproteins, Glycans, O-glycosylation, Proteome, Mass spectrometry

Eric CReynolds
contact affiliation	Melbourne Dental School, The University of Melbourne
contact email	e.reynolds@unimelb.edu.au
lab head
PaulVeith
contact affiliation	Melbourne Dental School
contact email	pdv@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040877
     1. Label: PRIDE project
     2. Name: Characterization of the O-Glycoproteome of Flavobacterium johnsoniae