PXD040873

PXD040873 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The Role of TGFbeta-regulated osteocytic perilacunar/canalicular remodeling in age-related bone fragility
Description	Poor bone quality (BQ) is a major factor in skeletal fragility in the elderly. Molecular mechanisms establishing and maintaining BQ, independent of bone mass, are unknown but are thought to be primarily determined by osteocytes. We hypothesize that the age-related decline in BQ results from suppression of osteocyte perilacunar/canalicular remodeling (PLR), which maintains bone material properties. We examined bones from young and aged mice with an osteocyte-intrinsic repression of TGFbeta signaling (TbetaRIIocy-/-) that suppresses PLR. Control-aged bone displayed decreased TGFbeta signaling and PLR, but aging male TbetaRIIocy-/- bone did not worsen existing PLR suppression. This epistatic relationship impacted collagen material behavior at the nano and tissue scale in macromechanical tests. The effects of age on bone mass, density, and mineral material behavior were independent of osteocytic TGFbeta. We determine that the decline of BQ with age arises from lost osteocyte function and maintenance of collagen integrity in a TGFbeta-dependent fashion.
HostingRepository	MassIVE
AnnounceDate	2023-11-03
AnnouncementXML	Submission_2023-11-03_15:57:36.592.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joanna Bons
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	3-hydroxy-L-proline; 4-hydroxy-L-proline
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-14 17:33:02	ID requested
⏵ 1	2023-11-03 15:57:36	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Bone proteomics, TGFbeta signaling, Data-independent acquisition (DIA), Quantification

submitter keyword: Bone proteomics, TGFbeta signaling, Data-independent acquisition (DIA), Quantification

Contact List

Birgit Schilling
contact affiliation	Buck Institute
contact email	bschilling@buckinstitute.org
lab head
Joanna Bons
contact affiliation	Buck Institute for Research on Aging
contact email	jbons@buckinstitute.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091488/