⮝ Full datasets listing

PXD040835

PXD040835 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEpigenetic mechanisms to propagate histone acetylation by p300/CBP
DescriptionHistone acetylation is important for the activation of gene transcription but little is known about its direct ‘read/write’ mechanisms. Here, we report cryo-electron microscopy structures in which a p300/CBP multidomain monomer recognizes histone H4 N-terminal tail (NT) acetylation (ac) in a nucleosome and acetylates non-H4 histone NTs within the same nucleosome. p300/CBP not only recognized H4NTac via the bromodomain pocket responsible for ‘reading’, but also interacted with the DNA minor grooves via the outside of that pocket. This directed the catalytic center of p300/CBP to one of the non-H4 histone NTs. The primary target that p300 ‘writes’ by ‘reading’ H4NTac was H2BNT, and H2BNTac promoted H2A-H2B dissociation from the nucleosome. We propose a model in which p300/CBP ‘replicates’ histone NT acetylation within the H3-H4 tetramer to inherit epigenetic storage, and ‘transcribes’ it from the H3-H4 tetramer to the H2B-H2A dimers to activate context-dependent gene transcription through local nucleosome destabilization.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:04:12.780.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD040835
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTakehiro Suzuki
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-13 17:58:47ID requested
12023-05-07 20:56:50announced
22023-07-24 03:05:37announced2023-07-24: Updated project metadata.
32023-11-14 09:04:20announced2023-11-14: Updated project metadata.
Publication List
Kikuchi M, Morita S, Wakamori M, Sato S, Uchikubo-Kamo T, Suzuki T, Dohmae N, Shirouzu M, Umehara T, Epigenetic mechanisms to propagate histone acetylation by p300/CBP. Nat Commun, 14(1):4103(2023) [pubmed]
10.6019/PXD040835;
Keyword List
submitter keyword: P300/CBP, Histone, Acetylation
Contact List
Naoshi Dohmae
contact affiliationBiomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact emaildohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliationRIKEN Center for Sustainable Resource Science
contact emailtakehirosuzuki@riken.jp
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD040835
PRIDE project URI
Repository Record List
[ + ]