PXD040835 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Epigenetic mechanisms to propagate histone acetylation by p300/CBP |
Description | Histone acetylation is important for the activation of gene transcription but little is known about its direct ‘read/write’ mechanisms. Here, we report cryo-electron microscopy structures in which a p300/CBP multidomain monomer recognizes histone H4 N-terminal tail (NT) acetylation (ac) in a nucleosome and acetylates non-H4 histone NTs within the same nucleosome. p300/CBP not only recognized H4NTac via the bromodomain pocket responsible for ‘reading’, but also interacted with the DNA minor grooves via the outside of that pocket. This directed the catalytic center of p300/CBP to one of the non-H4 histone NTs. The primary target that p300 ‘writes’ by ‘reading’ H4NTac was H2BNT, and H2BNTac promoted H2A-H2B dissociation from the nucleosome. We propose a model in which p300/CBP ‘replicates’ histone NT acetylation within the H3-H4 tetramer to inherit epigenetic storage, and ‘transcribes’ it from the H3-H4 tetramer to the H2B-H2A dimers to activate context-dependent gene transcription through local nucleosome destabilization. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:04:12.780.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD040835 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Takehiro Suzuki |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-13 17:58:47 | ID requested | |
1 | 2023-05-07 20:56:50 | announced | |
2 | 2023-07-24 03:05:37 | announced | 2023-07-24: Updated project metadata. |
⏵ 3 | 2023-11-14 09:04:20 | announced | 2023-11-14: Updated project metadata. |
Publication List
Kikuchi M, Morita S, Wakamori M, Sato S, Uchikubo-Kamo T, Suzuki T, Dohmae N, Shirouzu M, Umehara T, Epigenetic mechanisms to propagate histone acetylation by p300/CBP. Nat Commun, 14(1):4103(2023) [pubmed] |
10.6019/PXD040835; |
Keyword List
submitter keyword: P300/CBP, Histone, Acetylation |
Contact List
Naoshi Dohmae |
contact affiliation | Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN |
contact email | dohmae@riken.jp |
lab head | |
Takehiro Suzuki |
contact affiliation | RIKEN Center for Sustainable Resource Science |
contact email | takehirosuzuki@riken.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040835
- Label: PRIDE project
- Name: Epigenetic mechanisms to propagate histone acetylation by p300/CBP