PXD040806

PXD040806 is an original dataset announced via ProteomeXchange.

Title	DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC
Description	Hitherto, most studies on POLD1 have mainly focused on the effect of POLD1 inactivation mutation in tumors. Nonetheless, the mechanism underlying high POLD1 expression in tumorigenesis remains elusive. Herein, we substantiated the pro-carcinogenic role of POLD1 in bladder cancer (BLCA) and found that POLD1 expression is related to malignancy and prognosis of BLCA. Next, we demonstrated that POLD1 could promote proliferation and metastasis of BLCA via MYC. Mechanistically, we demonstrated that POLD1 was able to stabilize MYC in a manner independent of DNA polymerase activity. POLD1 attenuated the FBXW7-mediated ubiquitination degradation of MYC by directly binding to the MYC homology box 1 domain competitively with FBXW7. Moreover, we found that POLD1 can form a complex with MYC to promote the transcriptional activity of MYC. MYC could also transcriptionally activate POLD1, forming a POLD1-MYC positive feedback loop to enhance the pro-carcinogenic effect of POLD1-MYC on BLCA. Overall, our study suggests a novel MYC-driven mechanism for BLCA, and POLD1 has the potential as a biomarker for BLCA.
HostingRepository	PRIDE
AnnounceDate	2023-03-29
AnnouncementXML	Submission_2023-03-28_22:11:29.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YejinpengWang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-13 05:47:26	ID requested
⏵ 1	2023-03-28 22:11:29	announced

Dataset with its publication pending

submitter keyword: DNA polymerase δ (POLD1)

Bladder cancer (BLCA)

MYC

FBXW7

Ubiquitination degradation.

YuXiao
contact affiliation	Wuhan University
contact email	yu.xiao@whu.edu.cn
lab head
YejinpengWang
contact affiliation	Wuhan University
contact email	yejinpeng.wang@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD040806

PRIDE project URI

• PRIDE
  1. PXD040806
     1. Label: PRIDE project
     2. Name: DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC