PXD040804

PXD040804 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis for the study of Prn1 function, a protein similar to pirins. Identification and quantification of proteins whose expression changes in absence of Prn1 after hydrogen peroxide exposure.
Description	In C. albicans, after yeast-macrophage interaction up to 30% of yeast cells suffers oxidative stress mediated-apoptosis. In previous studies to reveal the response mechanisms to oxidative stress of this yeast we performed a quantitative proteomic analysis in cells exposed to hydrogen peroxide to evaluate changes in their proteome. In the analysis we identified a high increase in different oxidoreductase proteins like Oye32 but also in new undescribed proteins like Prn1, a protein similar to pirins. Pirins has already been described as a nuclear redox sensor in mammal cells which are implicated in oxidative stress response and as coregulator of different the transcription factors implicated in proliferation. Therefore, is probably that C. albicans Prn1 function will be related with oxidative stress response and also working as a regulator of different protein expression in response to this stress. In addition, prn1 lacks a homologue in S. cerevisiae, suggesting that it may be specific to pathogenic fungi, which makes important to study the role of this protein in the cell to finally be considered a possible drug target. In order to uncover the possible function of Prn1 in response to oxidative stress in C. albicans we carried out a quantitative proteomic assay in a wild type (SN250) and a prn1 strain treated with hydrogen peroxide. The main objective of the experiment was to identify which proteins decrease its abundance in prn1 cells in comparation with wild type cells in response to oxidative stress which means that their expression could be coregulated by Prn1. The second objective was to identify which proteins are overexpressed in response to oxidative stress to supply the lack or Prn1 which is very useful to identify new proteins implicated in oxidative stress response. Finally, these deceased and overexpressed proteins allowed us to identify which signalling pathways are regulated directly by Prn1 in response to oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-15_23:11:16.530.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040804
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-13 03:12:15	ID requested
⏵ 1	2024-06-15 23:11:17	announced

Arribas V, Monteoliva L, Hern, á, ez ML, Gil C, Molero G, Prn1 in the Oxidative Stress Response through a Proteomics Approach. Antioxidants (Basel), 13(5):(2024) [pubmed]

10.3390/antiox13050527;

10.6019/PXD040804;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: oxidative stress.,Quantitative proteomics, C. albicans, Prn1, Pirins

Concha Gil García
contact affiliation	Complutense University of Madrid
contact email	conchagil@ucm.es
lab head
Concha Gil
contact affiliation	UCM
contact email	cai.proteomica@pas.ucm.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040804
     1. Label: PRIDE project
     2. Name: Proteomic analysis for the study of Prn1 function, a protein similar to pirins. Identification and quantification of proteins whose expression changes in absence of Prn1 after hydrogen peroxide exposure.