PXD040793

PXD040793 is an original dataset announced via ProteomeXchange.

Title	The Drosophila Epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour.
Description	During Drosophila epithelial development, dynamic signalling filopodia (cytonemes) establish direct contacts between distant cells to facilitate the formation of the Hedgehog signalling gradient. However, not much is known about how cytonemes are regulated. In this study, we show that cytoneme dynamics and Hh signalling in the Drosophila epithelia depend on the Epidermal Growth Factor pathway and on its downstream effector Ras1. We describe that EGFR pathway is required to maintain in the wing disc epithelium the basal plasma membrane levels of Interference Hedgehog (Ihog), a critical Hh co-receptor and adhesion protein. Additionally, our data demonstrate that filamin A or Cheerio in Drosophila, responds to both Ihog and EGFR pathway and recruited to the basal site of the plasma membrane. This recruitment contributes to Ihog’s role in stabilizing cytonemes.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:15:18.672.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Citlali Gradilla
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-12 13:20:14	ID requested
⏵ 1	2025-05-06 09:15:19	announced

Lalioti VS, Gradilla AC, Jim, é, nez-Jim, é, nez C, Fern, á, ndez-Pardo C, S, á, nchez-Hern, á, ndez D, Aguirre-Tamaral A, S, á, nchez-Platero I, Jord, á, n-, À, lvarez S, Wakefield JG, Guerrero I, The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour. Nat Commun, 16(1):1994(2025) [pubmed]

10.1038/s41467-025-57162-5;

submitter keyword: Ihog, development.,Hedgehog signalling, EGFR, cytonemes

Isabel Guerrero
contact affiliation	Centro de Biología Molecular Severo Ochoa (CSIC)
contact email	iguerrero@cbm.csic.es
lab head
Ana Citlali Gradilla
contact affiliation	Centre of Molecular Biology Severo Ochoa (CSIC)
contact email	acgradilla@cbm.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD040793

PRIDE project URI

• PRIDE
  1. PXD040793
     1. Label: PRIDE project
     2. Name: The Drosophila Epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour.