PXD040785 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Myocardial proteome changes in aortic stenosis rats subjected to long-term aerobic exercise |
Description | The effects of exercise training (ET) on the heart of aortic stenosis (AS) rats are controversial and the mechanisms involved in alterations induced by ET have been poorly clarified. In this study we analyzed the myocardial proteome to identify proteins modulated by moderate intensity aerobic ET in rats with chronic supravalvar AS. Wistar rats were divided into four groups: sedentary control (C-Sed), exercised control (C-Ex), sedentary aortic stenosis (AS-Sed), and exercised AS (AS-Ex). ET consisted of five treadmill running sessions per week for 16 weeks. Statistical analysis was performed by ANOVA or Kruskal-Wallis and Goodman tests. Results were discussed at a significance level of 5%. At the end of the experiment, AS-Ex rats had higher functional capacity, lower blood lactate concentration, and better cardiac structural and left ventricular functional parameters than the AS-Sed. Myocardial proteome analysis showed that AS-Sed had higher protein expressions related to the glycolytic pathway, oxidative stress, and inflammation, and lower protein expressions related to beta-oxidation than C-Sed. AS-Ex had higher expression of one protein related to mitochondrial biogenesis and lower protein expressions associated with oxidative stress and inflammation than AS-Sed. Proteomic data were validated for proteins related to lipid and glycolytic metabolism. In conclusion, chronic pressure overload changes the expression of myocardial proteins that are mainly involved in lipid and glycolytic energy metabolism in rats. Moderate intensity aerobic training attenuates protein expressions related to oxidative stress and inflammation and increases protein expressions related to mitochondrial biogenesis. Protein changes are associated with improved functional capacity, cardiac remodeling and left ventricular function in aortic stenosis rats. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-29 |
AnnouncementXML | Submission_2024-02-29_08:21:21.326.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Licia C. Silva-Costa |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Xevo Q-Tof |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-10 21:21:30 | ID requested | |
⏵ 1 | 2024-02-29 08:21:21 | announced | |
Publication List
Keyword List
submitter keyword: supravalvular aortic stenosis, myocardium, ventricular dysfunction, rat, aerobic exercise training,Proteomics |
Contact List
Gustavo Augusto |
contact affiliation | Department of Internal Medicine, Botucatu Medical School, São Paulo State University, UNESP, SP, Brazil |
contact email | gustavo.mota@unesp.br |
lab head | |
Licia C. Silva-Costa |
contact affiliation | Unicamp |
contact email | l065387@g.unicamp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040785 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040785
- Label: PRIDE project
- Name: Myocardial proteome changes in aortic stenosis rats subjected to long-term aerobic exercise