PXD040781

PXD040781 is an original dataset announced via ProteomeXchange.

Title	Comparative, quantitative proteomic analysis of cell shape transitions in Haloferax volcanii
Description	Cell shape plays a crucial role in microbial survival. While Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on environmental conditions, little is known about mechanisms underpinning archaeal cell-shape determination. We identified mutants that exclusively form rods and carried out comprehensive proteomics studies in which we compared these mutants to previously identified disk-only mutant strains and wild-type. Using this approach, we identified several additional candidates for shape determination. The generation of deletion mutants lacking genes encoding potential rod- and disk-determining factors, HVO_2174 (RdfA) and HVO_2176 (DdfA), respectively, resulted in rod- and disk-defective phenotypes. Comprehensive proteomics performed with ∆rdfA and ∆ddfA on these shape mutants implicated a diverse set of proteins, including transporters, signaling components, and transducers, as important for cell shape determination. We also identified structural proteins including the H. volcanii actin homolog volactin (VolA), a previously unknown cytoskeletal element, required for disk-shape morphogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-02-17
AnnouncementXML	Submission_2024-02-17_11:43:42.595.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246; scientific name: Archaea; NCBI TaxID: NCBITaxon:2157;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 16:03:50	ID requested
⏵ 1	2024-02-17 11:43:43	announced

10.1038/s41467-024-45196-0;

Schiller H, Hong Y, Kouassi J, Rados T, Kwak J, DiLucido A, Safer D, Marchfelder A, Pfeiffer F, Bisson A, Schulze S, Pohlschroder M, Identification of structural and regulatory cell-shape determinants in Haloferax volcanii. Nat Commun, 15(1):1414(2024) [pubmed]

submitter keyword: Archaeal Proteome Project, Ursgal,Archaea, Haloferax volcanii, label-free quantification, cell shape

Mechthild Pohlschroder
contact affiliation	University of Pennsylvania, Department of Biology
contact email	pohlschr@sas.upenn.edu
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040781

PRIDE project URI

• PRIDE
  1. PXD040781
     1. Label: PRIDE project
     2. Name: Comparative, quantitative proteomic analysis of cell shape transitions in Haloferax volcanii