PXD040775

PXD040775 is an original dataset announced via ProteomeXchange.

Title	An optimized antibiotic from rhizobacteria against multidrug-resistant Gram-positive pathogens
Description	The rapid rise of antibiotic resistance and the urgent need for new antibiotics call for the unique skeleton of compounds with different mechanisms of action. Microbial natural products dominate the preferred chemical scaffolds for antibiotic discovery. A plant-microbial antibiotic is an appealing origin yet underexplored due to a dearth of comprehensive studies of antibacterial activity and structural characteristics. In this study, phloroglucinol derived isolated from the plant-root-associated bacterium Pseudomonas fluorescens was modified for structure–activity relationship study. 2,4-diproylphloroglucinol (DPPG) displayed bactericidal activity against a wide range of Gram-positive bacteria including multidrug-resistant pathogens. Interestingly, the bactericidal mechanism study exhibits that DPPG binds to type II NADH dehydrogenases (NDH-2) in the respiratory chain and disrupts the proton-motive force of S. aureus. We validated the efficacy of DPPG in vivo through mice models associated with infection or contamination. Our finding provides a novel antibiotic to treat S. aureus- associated infections and a potential target for antibiotic design and study.
HostingRepository	PRIDE
AnnounceDate	2025-02-15
AnnouncementXML	Submission_2025-02-15_01:21:22.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hanzhong feng
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 09:03:12	ID requested
⏵ 1	2025-02-15 01:21:22	announced

10.1016/J.CHEMBIOL.2024.06.017;

submitter keyword: DPPG,PMF,S.aureus,NDH-2

Yongxing He
contact affiliation	Lanzhou University
contact email	heyx@lzu.edu.cn
lab head
hanzhong feng
contact affiliation	Lanzhou University
contact email	fenghzh19@lzu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD040775

PRIDE project URI

• PRIDE
  1. PXD040775
     1. Label: PRIDE project
     2. Name: An optimized antibiotic from rhizobacteria against multidrug-resistant Gram-positive pathogens