PXD040773

PXD040773 is an original dataset announced via ProteomeXchange.

Title	Identification and PTPs in Anncaliia algerae
Description	This project is focused on isolation and characterization of polar tube proteins (PTPs) of Anncaliia algerae using diverse bioinformatic and experimental methodologies - from mass spectrometry to heterologous expression to antibody production and immunolocalization. A novel protein was isolated from A. algerae polar tube extracts named PTP6 which could participates in recognition of host cell receptors as well.Moreover the obtained data on immunolabeling of the polar tubes in the process of extrusion, strongly support one of three hypothetical mechanisms for spore firing, namely the “everting-like-a-glove finger” model. The practical result of this fundamental research is the elaboration of the novel workflow that allows identification and isolation of PTPs in a given microsporidian species wich unites different approaches, such as homology search, sporal protein extractions by thiol-reducing agents, antibody-based approaches, as well as a protocol for purification of extruded polar tubes combined with mass spectrometry to identify new polar tube components.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:24.687.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Didier VIALA
SpeciesList	scientific name: Anncaliia algerae; NCBI TaxID: 723287;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 07:52:33	ID requested
1	2023-07-20 11:41:45	announced
⏵ 2	2023-11-14 09:03:29	announced	2023-11-14: Updated project metadata.

Fayet M, Prybylski N, Collin ML, Peyretaillade E, Wawrzyniak I, Belkorchia A, Akossi RF, Diogon M, El Alaoui H, Polonais V, Delbac F, Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae. Sci Rep, 13(1):8773(2023) [pubmed]

submitter keyword: Microsporidia

Anncaliia algerae

Polar tube

Invasion

PTP

Frédéric DELBAC
contact affiliation	Université Clermont Auvergne, CNRS, Laboratoire Microorganismes : Génome et Environnement, F-63000 Clermont-Ferrand, France
contact email	frederic.delbac@uca.fr
lab head
Didier VIALA
contact affiliation	INRAE
contact email	didier.viala@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD040773

PRIDE project URI

• PRIDE
  1. PXD040773
     1. Label: PRIDE project
     2. Name: Identification and PTPs in Anncaliia algerae