PXD040772

PXD040772 is an original dataset announced via ProteomeXchange.

Title	Large scale screens of small molecules using multiple sources of human acute leukemia cells reveal novel targeting strategies
Description	Useful targeted therapeutics for high risk subgroups of acute leukemia remain an unmet medical need. Here we report the results of a large-scale screening strategy that tested thousands of compounds targeting many signalling pathways for their ability to inhibit the survival and growth in vitro of human leukemic cells from multiple sources; i.e., patients’ samples, de novo generated human leukemia models, and established human leukemic cell lines. The responses of the cells from the de novo models exhibited similar responses to those of the patients’ samples both of which showed similar differences with the cell-line responses. Analysis of differences in subtype-specific therapeutic vulnerabilities made possible by the scale of this screen enabled the identification of a new specific modulators of apoptosis, shikonin, exemplifying its power to identify a relevant mechanism of action of small molecules with a complex polypharmacology. In summary, the results create a new platform for uncovering new therapeutic options for high-risk human leukemias, in addition to reinforcing the importance of the test sample choice for effective drug discovery.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:15:36.707.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040772
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eric Bonneil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 06:48:24	ID requested
⏵ 1	2025-05-06 09:15:37	announced

10.6019/PXD040772;

10.1038/s41375-024-02400-w;

Safa-Tahar-Henni S, P, á, ez Martinez K, Gress V, Esparza N, Roques É, Bonnet-Magnaval F, Bilodeau M, Gagn, é V, Bresson E, Cardin S, El-Hachem N, Iasenza I, Alzial G, Boivin I, Nakamichi N, Soufflet AC, Mirela Pascariu C, Duchaine J, Mathien S, Bonneil É, Eppert K, Marinier A, Sauvageau G, Deblois G, Thibault P, H, é, bert J, Eaves CJ, Cellot S, Barab, é F, Wilhelm BT, Comparative small molecule screening of primary human acute leukemias, engineered human leukemia and leukemia cell lines. Leukemia, 39(1):29-41(2025) [pubmed]

submitter keyword: acute leukemia

Brian Wilhelm
contact affiliation	IRIC-Université de Montréal
contact email	brian.wilhelm@umontreal.ca
lab head
Eric Bonneil
contact affiliation	Proteomic Platform
contact email	eric.bonneil@umontreal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040772
     1. Label: PRIDE project
     2. Name: Large scale screens of small molecules using multiple sources of human acute leukemia cells reveal novel targeting strategies