PXD040764 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Venom Proteomics of Vespa velutina nigrithorax (Hym.: Vespidae) Queens and Workers: A Quantitative SWATH-MS Analysis |
| Description | Health risks caused by stings from Vespa velutina nigrithorax (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac based on Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). The study also performed proteomic quantitative analysis and examined the biological pathways and molecular functions of the proteins in the VS of VV gynes (i.e., future queens [SQ]) and workers [SW]). The total protein content per VS was significantly higher in the SW than in the SQ (274 ± 54 µg/sac vs. 175 ± 22 µg/sac; p=0.02). We quantified a total of 228 proteins belonging to 7 different classes: Insecta (n=191); Amphibia and Reptilia (n=20); Bacilli, γ-Proteobacteria and Pisoniviricetes (n=12); and Arachnida (n=5). Phage proteins of Paenibacillus larvae, the etiological agent of American foulbrood, and genome polyprotein from deformed wing virus were quantified by SWATH-MS. Among the 228 identified proteins, 66 showed significant differential expression between SQ and SW. The well-known allergens hyaluronidase A, venom antigen 5 and phospholipase A1 were significantly downregulated in the SQ venom. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_08:47:34.190.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Susana Bravo |
| SpeciesList | scientific name: Vespa velutina nigrithorax; NCBI TaxID: 202809; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-03-10 03:47:48 | ID requested | |
| 1 | 2023-05-10 14:28:18 | announced | |
| ⏵ 2 | 2023-11-14 08:47:35 | announced | 2023-11-14: Updated project metadata. |
Publication List
| Alonso-Sampedro M, Fe, á, s X, Bravo SB, Chantada-V, á, zquez MP, Vidal C, Venom Sac Queens and Workers: A Quantitative SWATH-MS Analysis. Toxins (Basel), 15(4):(2023) [pubmed] |
Keyword List
| submitter keyword: Asian hornet |
| Vespa velutina nigrithorax |
| Vespa spp. |
| Proteomics |
| SWATH-MS |
| Hymenopteran |
| Venom |
| Allergy. |
Contact List
| Susana Bravo |
|---|
| contact affiliation | Fundacion Instituto de Investigaciones Sanitarias de Santiago de Compostela |
| contact email | sbbravo@gmail.com |
| lab head | |
| Susana Bravo |
|---|
| contact affiliation | FIDIS |
| contact email | sbbravo@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040764
- Label: PRIDE project
- Name: Venom Proteomics of Vespa velutina nigrithorax (Hym.: Vespidae) Queens and Workers: A Quantitative SWATH-MS Analysis
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