PXD040760

PXD040760 is an original dataset announced via ProteomeXchange.

Title	Perinuclear Vimentin-RNF26 contacts integrate ER and endosomal responses to ER stress
Description	Compartmentalization of organelles in space and time affects their functional state and enables higher order regulation of essential cellular processes. How organellar residence is maintained in a defined area of the cell remains poorly understood. In this study, we uncover a new role for intermediate filaments in the maintenance of organellar architecture and dynamics, which is executed through a direct connection between Vimentin and the ER-embedded ubiquitin ligase ring finger protein 26 (RNF26). While the ubiquitin ligase function of RNF26 promotes perinuclear positioning of endolysosomes through binding endosomal adapters, catalytically inactive RNF26 preferentially binds Vimentin through a C-terminal motif. Loss of either RNF26 or Vimentin redistributes endolysosomes and ER membranes from the perinuclear ER towards the periphery. Furthermore, RNF26 and Vimentin control changes in ER morphology and invoke organelle compartmentalization during ER stress with consequences for stress-associated ER-phagy. Collectively, we define a new function for Vimentin-containing intermediate filaments as anchors of a dynamic interplay between the ER and endosomes, critical to the integrity of the perinuclear ER and corresponding perinuclear endosomal cloud during homeostatic and stress conditions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:57.569.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rayman Tjokrodirijo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 02:04:01	ID requested
1	2023-09-05 08:07:07	announced
⏵ 2	2023-11-14 09:04:58	announced	2023-11-14: Updated project metadata.

Cremer T, Voortman LM, Bos E, Jongsma ML, Ter Haar LR, Akkermans JJ, Talavera Orme, ñ, o CM, Wijdeven RH, de Vries J, Kim RQ, Janssen GM, van Veelen PA, Koning RI, Neefjes J, Berlin I, RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress. EMBO J, 42(18):e111252(2023) [pubmed]

submitter keyword: Vimentin, ER stress, endosomes, intermediate filaments, RNF26

Peter A. van Veelen
contact affiliation	Head Proteomics Group, Leiden University Medical Center, Center for Proteomics and Metabolomics, PO Box 9600, Postal zone P1-Q, 2300 RC Leiden, The Netherlands
contact email	P.A.van_Veelen@lumc.nl
lab head
Rayman Tjokrodirijo
contact affiliation	Leiden University Medical Center, Proteomics group
contact email	R.T.N.Tjokrodirijo@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD040760

PRIDE project URI

• PRIDE
  1. PXD040760
     1. Label: PRIDE project
     2. Name: Perinuclear Vimentin-RNF26 contacts integrate ER and endosomal responses to ER stress