PXD040748

PXD040748 is an original dataset announced via ProteomeXchange.

Title	MIRO-1 interacts with VDAC-1 to regulate mitochondrial membrane potential in Caenorhabditis elegans
Description	Precise regulation of mitochondrial fusion and fission is essential for cellular activity and animal development. Imbalances between these processes can lead to fragmentation and loss of normal membrane potential in individual mitochondria. In this study, we show that MIRO-1 is stochastically elevated in individual fragmented mitochondria and is required for maintaining mitochondrial membrane potential. We further observe a higher level of membrane potential in fragmented mitochondria in fzo-1 mutants and wounded animals. Moreover, MIRO-1 interacts with VDAC-1, a crucial mitochondrial ion channel located in the outer mitochondrial membrane, and this interaction depends on the residues E473 of MIRO-1 and K163 of VDAC-1. The E473G point mutation disrupts their interaction, resulting in a reduction of the mitochondrial membrane potential. Our findings suggest that MIRO-1 regulates membrane potential and maintains mitochondrial activity and animal health by interacting with VDAC-1. This study provides insight into the mechanisms underlying the stochastic maintenance of membrane potential in fragmented mitochondria.
HostingRepository	iProX
AnnounceDate	2023-05-10
AnnouncementXML	Submission_2023-07-13_20:05:45.853.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HengDa Zhou
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-09 18:51:42	ID requested
1	2023-05-09 23:32:57	announced
⏵ 2	2023-07-13 20:05:46	announced	2023-07-14: Update publication information.

Ren X, Zhou H, Sun Y, Fu H, Ran Y, Yang B, Yang F, Bjorklund M, Xu S, MIRO-1 interacts with VDAC-1 to regulate mitochondrial membrane potential in Caenorhabditis elegans. EMBO Rep, 24(8):e56297(2023) [pubmed]

submitter keyword: C. elegans, mitochondrial membrane potential, miro-1

Suhong Xu
contact affiliation	Zhejiang University
contact email	shxu@zju.edu.cn
lab head
HengDa Zhou
contact affiliation	Zhejiang University
contact email	11971012@zju.edu.cn
dataset submitter

iProX dataset URI