⮝ Full datasets listing

PXD040732

PXD040732 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTime-resolved N-terminome analysis of HCT116 cells during apoptosis
DescriptionProteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method that uses selective chemical derivatization of α-amines to isolate the N-terminal peptides. We applied LATE in combination with other N-terminomic method to study caspase-3 mediated proteolysis both in vitro and during apoptosis in cells. This enable us to identify many unreported caspase-3 cleavages, including some that cannot be identified by other methods. Furthermore, we find direct evidence that some of the caspase-3 generated neo-N-termini can be further modified by Nt-acetylation. This provides a global picture of the caspase-3 degradome and uncovers previously unrecognised functional crosstalk between post-translational Nt-acetylation and caspase proteolysis pathways.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:50:51.074.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOded Kleifeld
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdimethylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-09 11:45:57ID requested
12023-06-26 23:53:04announced
22023-11-14 09:01:58announced2023-11-14: Updated project metadata.
32024-10-22 05:50:51announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.mcpro.2023.100584;
Hanna R, Rozenberg A, Saied L, Ben-Yosef D, Lavy T, Kleifeld O, In-Depth Characterization of Apoptosis N-Terminome Reveals a Link Between Caspase-3 Cleavage and Posttranslational N-Terminal Acetylation. Mol Cell Proteomics, 22(7):100584(2023) [pubmed]
Keyword List
submitter keyword: caspase-3, N-terminal acetylation,N-terminome, apoptosis
Contact List
Oded Kleifeld
contact affiliationFaculty of Biology Technion-Israel Institute of Technology Haifa, Israel
contact emailokleifeld@technion.ac.il
lab head
Oded Kleifeld
contact affiliationTechnion
contact emailokleifeld@technion.ac.il
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD040732
PRIDE project URI
Repository Record List
[ + ]