PXD040708

PXD040708 is an original dataset announced via ProteomeXchange.

Title	HST-MRM-MS: A Novel High-Sample-Throughput Multiple Reaction Monitoring Mass Spectrometric Method for Multiplex Absolute Quantitation of Hepatocellular Carcinoma Serum Biomarker
Description	Absolute quantification of clinical biomarkers by mass spectrometry (MS) has been challenged due to low sample-throughput of current multiple reaction monitoring (MRM) methods. For this problem to be overcome, in this work, a novel high-sample-throughput multiple reaction monitoring mass spectrometric (HST-MRM-MS) quantification approach is developed to achieve simultaneous quantification of 24 samples. Briefly, triplex dimethyl reagents (L, M, and H) and eight-plex iTRAQ reagents were used to label the N- and C-termini of the Lys C-digested peptides, respectively. The triplex dimethyl labeling produces three coelute peaks in MRM traces, and the iTRAQ labeling produces eight peaks in MS2, resulting in 24 (3×8) channels in a single experiment. HST-MRM-MS has shown good accuracy (R2 > 0.98 for absolute quantification), reproducibility (RSD < 15%), and linearity (2–3 orders of magnitude). Moreover, the novel method has been successfully applied in quantifying serum biomarkers in hepatocellular carcinoma (HCC)-related serum samples. In conclusion, HST-MRM-MS is an accurate, high-sample-throughput, and broadly applicable MS-based absolute quantification method.
HostingRepository	iProX
AnnounceDate	2020-02-23
AnnouncementXML	Submission_2023-03-08_23:28:49.185.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaohui Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	QTRAP 6500+

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-08 23:28:42	ID requested
⏵ 1	2023-03-08 23:28:49	announced

Jiang H, Zhang L, Zhang Y, Xie L, Wang Y, Lu H, HST-MRM-MS: A Novel High-Sample-Throughput Multiple Reaction Monitoring Mass Spectrometric Method for Multiplex Absolute Quantitation of Hepatocellular Carcinoma Serum Biomarker. J Proteome Res, 18(1):469-477(2019) [pubmed]

submitter keyword: quantification clinical biomarker multiple reaction monitoring dimethyl labeling isobaric tags for relative and absolute quantification

Pengyuan Yang
contact affiliation	Fudan university
contact email	pyyang@fudan.edu.cn
lab head
Xiaohui Liu
contact affiliation	Fudan University
contact email	liuxiaohui@fudan.edu.cn
dataset submitter

iProX dataset URI