PXD040702

PXD040702 is an original dataset announced via ProteomeXchange.

Title	Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins
Description	Phytochrome B (phyB) is a plant photoreceptor that forms a membraneless organelle called a photobody. However, its constituents are not fully known. Here, we isolated phyB photobodies from Arabidopsis leaves using fluorescence-activated particle sorting and analyzed their components. We found that a photobody comprises ~1,500 phyB dimers along with other proteins that could be classified into two groups: The first includes proteins that directly interact with phyB and localize to the photobody when expressed in protoplasts, while the second includes proteins that interact with the first group proteins and require co-expression of a first-group protein to localize to the photobody. As an example of the second group, TOPLESS (TPL) interacts with PHOTOPERIODIC CONTROL OF HYPOCOTYL 1 (PCH1) and localizes to the photobody when co-expressed with PCH1. Mutations in TPL family members cause shortened hypocotyls under red light, demonstrating that the method identifies photobody components that regulate red light signaling. Together, our results support that phyB photobodies include not only phyB and its primary interacting proteins but also its secondary interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:38.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ga Seul Lee
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-08 19:24:01	ID requested
1	2023-04-03 07:33:28	announced
⏵ 2	2023-11-14 08:44:45	announced	2023-11-14: Updated project metadata.

Kim C, Kwon Y, Jeong J, Kang M, Lee GS, Moon JH, Lee HJ, Park YI, Choi G, Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins. Nat Commun, 14(1):1708(2023) [pubmed]

submitter keyword: Phytochrome, Photobody

Giltsu Choi
contact affiliation	Department of Biological Sciences, KAIST
contact email	gchoi@kaist.ac.kr
lab head
Ga Seul Lee
contact affiliation	Korea Research Institute of Bioscience & Biotechnology (KRIBB)
contact email	rktmf1013@kribb.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040702
     1. Label: PRIDE project
     2. Name: Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins