PXD040698

PXD040698 is an original dataset announced via ProteomeXchange.

Title	The proteome and phosphoproteome uncovers candidate proteins associated with vacuolar phosphate signal multipled by Vacuolar phosphate transporter1 (VPT1) in Arabidopsis
Description	Plant vacuoles serve as the primary intracellular compartments for inorganic phosphate (Pi) storage. Passage of Pi across vacuolar membranes plays a critical role in buffering the cytoplasmic Pi level against fluctuations of external Pi and metabolic activities. To gain new insights into the proteins and processes vacuolar Pi level regulated by Vacuolar phosphate transporter1 (VPT1) in Arabidopsis, we carried out TMT labeling proteome and phosphoproteome profiling of Arabidopsis wild-type (WT) and vpt1 loss-of-function mutant plants. The vpt1 mutant had a reduced vacuolar Pi level, but an increased cytosol Pi level. The mutant was stunted as reflected in the reduction of the fresh weight compared with WT plants, and bolting earlier under normal growth conditions in soil. Over 5566 proteins and 7965 phosphopeptides were quantified. About 146 and 83 proteins were significantly changed at protein abundance or site-specific phosphorylation levels, but only 6 proteins were shared between them. Functional enrichment analysis revealed that Pi starvation signal in Arabidopsis vacuole is associated with photosynthesis, translation, RNA splicing, and defense response, consistent with similar studies in Arabidopsis. Except for PAP26, EIN2, and KIN10, which are reported to be associated with phosphate starvation signal, we also found many differential proteins involved in abscisic acid (ABA) signaling, such as CARK1, SnRK1, and AREB3, were changed in response to vacuolar Pi starvation. Our study illuminates several new aspects of the phosphate starvation response and identifies important targets for further investigation and potential crop improvement.
HostingRepository	iProX
AnnounceDate	2023-03-08
AnnouncementXML	Submission_2023-05-29_19:57:54.048.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanjun Zhang
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-08 18:18:09	ID requested
1	2023-03-08 18:18:17	announced
⏵ 2	2023-05-29 19:57:54	announced	2023-05-30: Update publication information.

Zhang Y, Chen X, Feng J, Shen Y, Huang Y, The Proteome and Phosphoproteome Uncovers Candidate Proteins Associated With Vacuolar Phosphate Signal Multipled by Vacuolar Phosphate Transporter 1 (VPT1) in Arabidopsis. Mol Cell Proteomics, 22(6):100549(2023) [pubmed]

submitter keyword: Vacuolar phosphate transporter1, proteome, phosphoproteome, Pi starvation response

Yun Huang
contact affiliation	Beijing University of Agriculture
contact email	yunhuang@bua.edu.cn
lab head
Yanjun Zhang
contact affiliation	Beijing University of Agriculture
contact email	Z18330625012@163.COM
dataset submitter

iProX dataset URI