PXD040686

PXD040686 is an original dataset announced via ProteomeXchange.

Title	VSG Exclusion Protein 2 (VEX2) affinity purification combined with LC-MS/MS analysis in Trypanosoma brucei
Description	UPF1-like helicases play roles in telomeric heterochromatin formation and X-chromosome inactivation and we focus here on variant surface glycoprotein (VSG) exclusion-factor-2 (VEX2), a UPF1-related protein in the African trypanosome. This bloodstream parasite evades host immunity by employing monogenic and switchable VSG expression, a form of antigenic variation which involves transcription of one telomeric VSG gene at a time. A trans-splicing compartment and the single VSG transcription factory are juxtaposed and enriched for VEX1 and VEX2, respectively, but our understanding of the VSG exclusion mechanism remains otherwise incomplete. Here, we sought VEX2-interacting partners, using cryomilling and affinity purification followed my LC-MS/MS analysis. We confirmed previous observations that VEX2 interacts with VEX1 and CAF-1, but also found evidence for weaker interactions and/or spatial proximity to telomere binding proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:14:58.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Tinti
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-08 05:05:24	ID requested
1	2023-11-20 05:09:43	announced
⏵ 2	2024-10-22 06:14:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: VEX2,Trypanosoma brucei, cryomilling, LC-MS/MS

Joana R. Correia Faria
contact affiliation	Department of Biology University of York Heslington York YO10 5DD
contact email	joana.correiafaria@york.ac.uk
lab head
Michele Tinti
contact affiliation	Dundee University
contact email	m.tinti@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040686
     1. Label: PRIDE project
     2. Name: VSG Exclusion Protein 2 (VEX2) affinity purification combined with LC-MS/MS analysis in Trypanosoma brucei