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PXD040685

PXD040685 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn unexpected histone-binding activity for the MIER1 histone deacetylase complex suggests a potential chaperone function.
DescriptionHistone deacetylases 1 and 2 (HDAC1/2) serve as the catalytic subunit of six distinct families of nuclear complexes. These complexes repress gene transcription through removing acetyl groups from lysine residues in histone tails. In addition to the deacetylase subunit, these complexes typically contain transcription factor and/or chromatin binding activities. The MIER:HDAC complex has hitherto been poorly characterized. Here we show that MIER1 unexpectedly co-purifies with an H2A:H2B histone dimer. We show that MIER1 is also able to bind a complete histone octamer. Intriguingly, we found that a larger MIER1:HDAC1:BAHD1:C1QBP complex additionally co-purifies with an intact nucleosome on which H3K27 is either di- or tri-methylated. Together this suggests that the MIER1 complex acts downstream of PRC2 to expand regions of repressed chromatin and could potentially deposit histone octamer onto nucleosome-depleted regions of DNA.
HostingRepositoryPRIDE
AnnounceDate2023-04-14
AnnouncementXMLSubmission_2023-04-14_07:05:05.593.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarkCollins
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; acetylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-08 03:50:59ID requested
12023-04-14 07:05:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: methylation, deacetylase,Histone
Contact List
MarkCollins
contact affiliationSchool of Biosciences, University of Sheffield.
contact emailmark.collins@sheffield.ac.uk
lab head
MarkCollins
contact affiliationUniversity of Sheffield
contact emailmark.collins@sheffield.ac.uk
dataset submitter
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Dataset FTP location
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