PXD040678
PXD040678 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Dysregulations in the levels of lactate dehydrogenase and pyruvate kinase upon IL-17A and echinomycin treatments in human primary keratinocytes |
| Description | IL-17A driven hyperproliferation is a hallmark of psoriatic keratinocytes. The pathway analysis of shotgun proteomics data from human primary keratinocytes (HPKs) treated with IL-17A highlighted the role of increased levels of lactate dehydrogenase (LDH-A) and pyruvate kinase (PKM). We sought to validate these findings using multiple reaction monitoring (MRM) for LDH-A (6 peptides) and PKM (8 peptides). Moreover, speculating the role of the transcription factor HIF1a in regulation of LDH-A and PKM levels, we also probed these proteins upon inhibiting HIF1a activity with echinomycin in presence of IL-17A. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2024-08-01 |
| AnnouncementXML | Submission_2024-08-01_11:11:45.527.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Medha Gayathri J Pai |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | TSQ Altis |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-03-07 22:29:43 | ID requested | |
| ⏵ 1 | 2024-08-01 11:11:45 | announced |
Publication List
| Dhamija B, Marathe S, Sawant V, Basu M, Attrish D, Mukherjee D, Kumar S, Pai MGJ, Wad S, Sawant A, Nayak C, Venkatesh KV, Srivastava S, Barthel SR, Purwar R. IL-17A Orchestrates Reactive Oxygen Species/HIF1α-Mediated Metabolic Reprogramming in Psoriasis. J Immunol. 2024 Jan 15;212(2):302-316. doi: 10.4049/jimmunol.2300319. PMID: 38019129 |
| PMCID: PMC11100423. |
Keyword List
| submitter keyword: keratinocytes, Interleukin-17A, echinomycin, lactate dehydrogenase, pyruvate kinase |
Contact List
| Sanjeeva Srivastava | |
|---|---|
| contact affiliation | BSBE, IIT Bombay |
| contact email | sanjeeva@iitb.ac.in |
| lab head | |
| Medha Gayathri J Pai | |
| contact affiliation | BSBE, IIT Bombay |
| contact email | mgjpai@gmail.com |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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