PXD040665

PXD040665 is an original dataset announced via ProteomeXchange.

Title	IPD3, a transcription factor necessary to establish arbuscular mycorrhizal symbiosis affects immunity and metabolism of non-host Arabidopsis 65 million years after its evolutionary loss
Description	The goal of this study was to detect and quantify the presence of transgenic proteins in transformed A. thaliana plants and overall gene expression responses to presence of IPD3. Experimental plants were transformed with 3 versions of the gene Interacting Protein of DMI3 (IPD3), a transcription factor necessary for mycorrhizal symbiosis, derived from Medicago truncatula. Col-0 ecotype plants not transformed with any gene are labeled “WT.” Plants expressing the sequence of Medicago truncatula IPD3 with removal of introns but no coding sequence modifications are labeled “MtIPD3”. Plants expressing the coding sequence of IPD3 with an alteration causing a serine at position 50 to be replaced by aspartic acid, which results in consitutive activation via phosphomimicking, are labeled “S50DIPD3 .” Plants transformed with a truncated version of IPD3 consisting solely of the C-terminal DNA-binding domain found from aa 254-513, which has constitutively active DNA-binding activity, are labeled “IPD3-Min.” For shotgun proteomics, five biological replicates were included for each treatment. For targeted proteomics, one biological replicate was included for each treatment. Whole root or shoot tissue from plants grown for 5 weeks on ½ strength MS medium was harvested and immediately flash frozen with liquid nitrogen. This tissue was stored at -80ºC until it was ground to a fine powder under liquid nitrogen. The tissue was then weighed into 200 mg aliquots. Amino acid sequences of the respecitive transgenic proteins were used as a reference and “Wt” control plants were used as a negative control.
HostingRepository	PRIDE
AnnounceDate	2024-02-20
AnnouncementXML	Submission_2024-02-20_13:21:11.199.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simina Vintila
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-07 16:30:25	ID requested
⏵ 1	2024-02-20 13:21:11	announced

10.1007/S11103-024-01422-3;

submitter keyword: symbiosis, immunity, transformed plant, mycorrhizae, defense,transgenic proteins

Manuel Kleiner
contact affiliation	PMB, North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Simina Vintila
contact affiliation	North Carolina State University
contact email	svintil@ncsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040665

PRIDE project URI

• PRIDE
  1. PXD040665
     1. Label: PRIDE project
     2. Name: IPD3, a transcription factor necessary to establish arbuscular mycorrhizal symbiosis affects immunity and metabolism of non-host Arabidopsis 65 million years after its evolutionary loss