PXD040663

PXD040663 is an original dataset announced via ProteomeXchange.

Title	Identifying Targets of Pyrvinium Pamoate in Jurkat Cells using PISA
Description	Pyrvinium pamoate, an anthelmintic drug, inhibits mitochondrial biogenesis during initial T-cell activation; however, its molecular target(s) and mechanism of action are unknown. In this study, we treated Jurkat T cell lysate with pyrvinium pamoate (5 nM and 50μM) and used proteome integral solubility alteration (PISA), to identify potential targets.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:49:31.175.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brandon Gassaway
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-07 10:17:06	ID requested
1	2023-08-28 19:46:58	announced
⏵ 2	2023-11-14 07:49:31	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mitochondria, Pyrvinium Pamoate, Jurkat T cells, PISA

Steven Gygi
contact affiliation	Harvard Medical School, Department of Cell Biology
contact email	steven_gygi@hms.harvard.edu
lab head
Brandon Gassaway
contact affiliation	Harvard Medical School
contact email	brandon_gassaway@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD040663

PRIDE project URI

• PRIDE
  1. PXD040663
     1. Label: PRIDE project
     2. Name: Identifying Targets of Pyrvinium Pamoate in Jurkat Cells using PISA