PXD040649

PXD040649 is an original dataset announced via ProteomeXchange.

Title	Detection of Lactococcus lactis contamination in standard and ethanol-washed casein used in mouse diets for microbiome studies
Description	Casein is a common source of dietary protein in defined diets used to investigate the effect of diet on the gut microbiota. Previously it has been shown that the metagenomes of mice fed with a diet containing casein were contaminated with DNA from the bacterium Lactococcus lactis and that washing the casein with ethanol removed this contamination (https://doi.org/10.1016/j.chom.2019.06.013). We have identified the same phenomenon when identifying proteins from mouse diets with casein However, in contrast to DNA sequencing we did not see a reduction in L. lactis contamination in proteomes. Two lots each of standard casein and ethanol-washed casein were analyzed using proteomics.
HostingRepository	PRIDE
AnnounceDate	2024-12-18
AnnouncementXML	Submission_2024-12-18_12:11:12.792.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	J. Alfredo Blakeley Ruiz
SpeciesList	scientific name: Lactococcus lactis; NCBI TaxID: 1358; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-07 03:00:07	ID requested
⏵ 1	2024-12-18 12:11:13	announced

10.1101/2023.12.07.570621;

submitter keyword: microbiome,Mouse diets, casein, microbiota, ethanol wash, metaproteomics, dietary protein

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
J. Alfredo Blakeley Ruiz
contact affiliation	North Carolina State University, Raliegh, NC
contact email	jablakel@ncsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD040649

PRIDE project URI

• PRIDE
  1. PXD040649
     1. Label: PRIDE project
     2. Name: Detection of Lactococcus lactis contamination in standard and ethanol-washed casein used in mouse diets for microbiome studies