PXD040645

PXD040645 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis revealed the potential role of MAGE-D2 in therapeutic targeting of triple-negative breast cancer
Description	Triple negative breast cancer (TNBC) is one of the most aggressive molecular subtypes in breast cancer. Due to the lack of effective therapeutic targets, the clinical outcomes for TNBC patients remain poor. Here, we performed an integrated proteomic analysis including bioinformatic analysis of proteome database, label-free proteomics, and immunoprecipitation coupled with mass spectrometry (IP-MS) to explore potential therapeutic targets for TNBC. Bioinformatic analysis showed that MAGE-D2 was overexpressed in TNBC. In vivo and in vitro experiments indicated that MAGE-D2 overexpression could promote TNBC proliferation and metastasis. In addition, label-free proteomics revealed that MAGE-D2 played a cancer-promoting role by activating PI3K/AKT pathway. Moreover, the results of IP-MS and cross-linking/xIP-MS demonstrated that MAGE-D2 could interact with Hsp70 and prevent Hsp70 degradation. Therefore, MAGE-D2 could be a potential therapeutic target for TNBC, and inhibiting MAGE-D2 may have important therapeutic relevance.
HostingRepository	iProX
AnnounceDate	2023-03-07
AnnouncementXML	Submission_2024-02-28_18:52:27.663.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyu Shi
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-07 00:04:01	ID requested
1	2023-03-07 00:04:14	announced
⏵ 2	2024-02-28 18:52:28	announced	2024-02-29: Update information.

Shi X, Liu C, Zheng W, Cao X, Li W, Zhang D, Zhu J, Zhang X, Chen Y, Proteomic Analysis Revealed the Potential Role of MAGE-D2 in the Therapeutic Targeting of Triple-Negative Breast Cancer. Mol Cell Proteomics, 23(1):100703(2024) [pubmed]

submitter keyword: MAGE-D2, Proteomic analysis, Triple negative breast cancer, Potential therapeutic target, Hsp70

Yun Chen
contact affiliation	Nanjing Medical University
contact email	ychen@njmu.edu.cn
lab head
Xiaoyu Shi
contact affiliation	Nanjing Medical University
contact email	shixy@njmu.edu.cn
dataset submitter

iProX dataset URI