PXD040617

PXD040617 is an original dataset announced via ProteomeXchange.

Title	The Glomerulus Multi-Omics Analysis Provides Deeper Insights into Diabetic Nephropathy
Description	Diabetic nephropathy (DN) is the leading cause of end-stage renal disease (ESRD), however, the detail mechanisms remain unclear. Here, we integrated the transcriptomics and proteomics profiles of glomeruli isolated from 50 biopsy-proven DN patients and 25 controls to explore the new discoveries underlying the pathogenesis of DN. First, 1,152 genes were differentially expressed at mRNA or protein level, and 364 of them showed strong correlation. Those strong correlated genes were divided into four different functional modules, including energy metabolism processes, extracellular matrix organization and cell adhesion, mRNA metabolism and protein translation, immune system and inflammation. In addition, a transcription factors (TFs) - target genes (TGs) regulatory network was constructed, among which 30 TFs up-regulated at protein levels and 265 downstream TGs were differentially expressed at the mRNA levels. Those TFs constitute the integration centers of different signal transduction pathways, and have great therapeutic potential in controlling the abnormal expression of TGs and the pathological process of DN. Furthermore, 29 new DN-specific splice-junction peptides with high confidence were identified, which may exert novel roles in the pathological progression of DN. So, our in-depth integrative transcriptomic-proteomic analysis provided deeper insights into the pathogenesis of DN and opened the potential avenue for finding new therapeutic interventions in DN.
HostingRepository	iProX
AnnounceDate	2023-03-06
AnnouncementXML	Submission_2023-09-11_20:57:33.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tingting Zhao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	N-acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-06 04:46:26	ID requested
⏵ 1	2023-09-11 20:57:34	announced

Zhao T, Zhan D, Qu S, Jiang S, Gan W, Qin W, Zheng C, Cheng F, Lu Y, Liu M, Shi J, Liang H, Wang Y, Qin J, Zen K, Liu Z, Transcriptomics-proteomics Integration reveals alternative polyadenylation driving inflammation-related protein translation in patients with diabetic nephropathy. J Transl Med, 21(1):86(2023) [pubmed]

submitter keyword: Diabetic nephropathy, glomeruli, transcriptomics, proteomics, integration

Zhihong Liu
contact affiliation	National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine
contact email	liuzhihong@nju.edu.cn
lab head
Tingting Zhao
contact affiliation	National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu.
contact email	zhaoting19900912@163.com
dataset submitter

iProX dataset URI