PXD040605

PXD040605 is an original dataset announced via ProteomeXchange.

Title	Complementary transcriptomic and proteomic analyses reveal the inhibitory effect of deoxynivalenol on enteric smooth muscle cells contractility via regulating the ECM-integrin-actin polymerization signaling pathway
Description	Deoxynivalenol (DON) is one of the frequent Fusarium mycotoxins and poses a serious threat to public health worldwide, especially in humans and pigs. Growth retardation is the critical symptom of DON poisoning, commonly manifested by weight loss and reduced food intake. The gastrointestinal tract (GIT) is the principal target organ in the body and DON-induced weight loss is tightly connected with its capacity to reduce feed intake by disturbing the GIT motility. Our previous reports for the first time indicated that DON interfered with intestinal motility by injuring the contractility of enteric smooth muscle cells (SMC), suggesting that enteric SMC might be a main target in the procession of DON-induced GIT motility disorder. However, underlying molecular mechanisms of DON-induced enteric SMC contractile dysfunction remain poorly understood until now. Here, we explored the potential molecular mechanisms by employing a complementary method of transcriptomics and proteomics using the porcine enteric smooth muscle cell line (PISMC) as an experimental model. First, the DON-induced contractile dysfunction of the enteric SMC model was successfully established with PISMC, which was evidenced by decreased cell contractility, cell viability, and the expression levels of SMC contractile markers (contractile apparatus components). Subsequently, transcriptomic and proteomic analyses were used to reveal the differentially expressed gene and protein profiles after DON exposure. A total of 2944 genes and 980 proteins were identified as differentially expressed. Additionally, a systematic bioinformatic analysis of gene-protein interactions revealed that a total of 152 co-differentially expressed genes were identified. The data uncover that the expression of numerous extracellular matrix (ECM) proteins was downregulated in PISMC under DON exposure. DON also remarkably decreased the expression of multiple integrin subunits, resulting in the inhibition of the ECM-integrin receptor interaction and its mediated signaling. Furthermore, DON treatment could depress actin polymerization, as reflected by the upregulated expression of Rho GTPase-activating proteins, cofilin and the downregulated expression levels of Rho guanine nucleotide exchange factors in PISMC. Meanwhile, the expression levels of downstream target genes that were responsible for sustaining contractile phenotype were significantly inhibited in PISMC challenge with DON, including actin alpha 2, caldesmon 1, and myosin regulatory light chain 9 and so on. Taken together, the current results suggest that DON inhibits enteric smooth muscle cell contractility via regulating the ECM-integrin-actin polymerization signaling pathway mediated downstream contractile apparatus genes expression. Our findings provide novel insights into potential mechanisms behind the DON toxicological effects in the GIT of humans and animals.
HostingRepository	iProX
AnnounceDate	2023-03-06
AnnouncementXML	Submission_2024-01-03_20:05:41.106.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Qiao
SpeciesList	scientific name: Sus scrofa domesticus; NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-05 22:54:22	ID requested
⏵ 1	2024-01-03 20:05:41	announced

Dataset with its publication pending

submitter keyword: Deoxynivalenol, Enteric smooth muscle contractility, ECM-receptor interaction, Actin polymerization, Transcriptomic, Proteomic

Wen Yao
contact affiliation	Nanjing Agricultural University
contact email	yaowen67jp@njau.edu.cn
lab head
Yu Qiao
contact affiliation	Nanjing Agricultural University
contact email	849779152@qq.com
dataset submitter

iProX dataset URI