PXD040597

PXD040597 is an original dataset announced via ProteomeXchange.

Title	Identification of inhibitors for the trans-membrane Trypanosoma cruzi eIF2alpha kinase relevant for parasite proliferation
Description	We studied the impact of the gene knockout of the TcK2 protein kinase of Trypanosoma cruzi, the causative agent of Chagas disease. TcK2 is structurally similar to the human kinase PERK, which phosphorylates the initiation factor eIF2alpha, and in turn, inhibits translation initiation. As TcK2 kinase promotes parasite proliferation within mammalian cell, it is a potential target for treatment of Chagas disease. Proteomics indicates that proliferative forms express genes including trans-sialidases, normally restricted to infective and non-proliferative trypomastigotes. In addition, TcK2 knockout cells lose phosphorylation of eukaryotic initiation factor 3 and cyclic AMP responsive-like element, recognized to promote growth, likely explaining both decreased proliferation and augmented differentiation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:56:41.070.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Schenkman
SpeciesList	scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-05 08:11:14	ID requested
1	2023-07-20 11:50:56	announced
2	2023-11-14 09:03:42	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:56:44	announced	2024-10-22: Updated project metadata.

10.1016/j.jbc.2023.104857;

Marcelino TP, Fala AM, da Silva MM, Souza-Melo N, Malvezzi AM, Klippel AH, Zoltner M, Padilla-Mejia N, Kosto S, Field MC, Burle-Caldas GA, Teixeira SMR, Cou, ñ, ago RM, Massirer KB, Schenkman S, kinase relevant for parasite proliferation. J Biol Chem, 299(7):104857(2023) [pubmed]

submitter keyword: chemical inhibitor, recombinant protein, T. cruzi EIF2AK2,Protein kinase, Chagas disease, invasion, proteome, assay eIF2alpha

Sergio Schenkman
contact affiliation	Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, Universidade Federal de Sao Paulo
contact email	sschenkman@unifesp.br
lab head
Sergio Schenkman
contact affiliation	Universidade Federal de São Paulo
contact email	sschenkman@unifesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040597
     1. Label: PRIDE project
     2. Name: Identification of inhibitors for the trans-membrane Trypanosoma cruzi eIF2alpha kinase relevant for parasite proliferation