PXD040567 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Data-dependent acquisition based proteomic analysis of Mycobacterium fortuitum ATCC 6841 |
Description | Detection of species-specific proteotypic peptides for accurate and easy characterization of infectious non-tuberculous mycobacteria such as Mycobacterium fortuitum is essential. Therefore, we carried out an in-depth global proteomic experiment using M. fortuitum ATCC 6841 strain followed by a proteome database search and spectral library generation. The lysate was subjected to in-solution proteomic sample preparation and fractionated using an offline C18 StageTip. Each fraction was acquired in technical triplicates using a 180 min data-dependent acquisition (DDA) method in Orbitrap Fusion Tribrid (Thermo Scientific) mass spectrometer. The resulting raw DDA data were searched against the M. fortuitum proteome database using Proteome Discoverer and FragPipe. The resulting peptide spectrum matches were converted into a spectral library using BiblioSpec. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-07 |
AnnouncementXML | Submission_2024-03-06_22:22:32.440.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Keshava Prasad T. S. |
SpeciesList | scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Mycolicibacterium fortuitum subsp. fortuitum DSM 46621 = ATCC 6841; NCBI TaxID: 1214102; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-03 01:26:48 | ID requested | |
⏵ 1 | 2024-03-06 22:22:33 | announced | |
Publication List
10.1021/acs.jproteome.3c00850; |
Kotimoole CN, Ramya VK, Kaur P, Reiling N, Shandil RK, Narayanan S, Flo TH, Prasad TSK, Discovery of Species-Specific Proteotypic Peptides To Establish a Spectral Library Platform for Identification of Nontuberculosis Mycobacteria from Mass Spectrometry-Based Proteomics. J Proteome Res, 23(3):1102-1117(2024) [pubmed] |
Keyword List
submitter keyword: Mycobacterium fortuitum, non-tuberculous mycobacteria, DDA, LC-MS/MS |
Contact List
T. S. Keshava Prasad |
contact affiliation | Professor and Deputy Director, Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Deralakatte, Mangalore 575018, India |
contact email | keshav@yenepoya.edu.in |
lab head | |
Keshava Prasad T. S. |
contact affiliation | Institute of Bioinformatics, Bangalore, Karnataka, India. |
contact email | keshav@ibioinformatics.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040567
- Label: PRIDE project
- Name: Data-dependent acquisition based proteomic analysis of Mycobacterium fortuitum ATCC 6841