PXD040559

PXD040559 is an original dataset announced via ProteomeXchange.

Title	Glucose depletion enables Candida albicans mating independent of the epigenetic white-opaque switch
Description	The human fungal pathogen Candida albicans can switch stochastically and heritably between a “white” phase and an “opaque” phase. Opaque cells are the mating-competent form of the species whereas white cells are essentially “sterile”. Here, we report that glucose depletion, a common nutrient stress, enables C. albicans white cells to undergo efficient sexual mating. The relative expression levels of pheromone-sensing and mating-associated genes (including STE2/3, MFA1, MFalpha1, FIG1, FUS1, and CEK1/2) were increased under glucose depletion conditions, while expression of mating repressors TEC1 and DIG1 was decreased. We show that Cph1 and Tec1, factors that act downstream of the pheromone MAPK pathway, play opposite roles in regulating white cell mating as TEC1 deletion or CPH1 overexpression promoted white cell mating. Moreover, inactivation of the Cph1 repressor Dig1 increased white cell mating ~4,000 fold in glucose-depleted medium relative to that in the presence of glucose. These findings reveal that the white-to-opaque epigenetic switch may not be a prerequisite for sexual mating in C. albicans in nature. Given parallels between C. albicans white cell mating to that of other yeast species, this mechanism of mating could represent a more ancient strategy of sexual reproduction in C. albicans.
HostingRepository	iProX
AnnounceDate	2023-03-03
AnnouncementXML	Submission_2023-04-18_19:21:40.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Li
SpeciesList	scientific name: Candida albicans; NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-02 18:02:06	ID requested
1	2023-03-02 18:02:20	announced
⏵ 2	2023-04-18 19:21:41	announced	2023-04-19: Update publication information.

Guan G, Tao L, Li C, Xu M, Liu L, Bennett RJ, Huang G, the epigenetic white-opaque switch. Nat Commun, 14(1):2067(2023) [pubmed]

submitter keyword: Candida albicans, mating projection, sexual reproduction, phenotypic switching, Cph1, MAPK pathway, Tec1

Guanghua Huang
contact affiliation	Fudan University
contact email	78439251@qq.com
lab head
Tao Li
contact affiliation	Fudan University
contact email	taoli@fudan.edu.cn
dataset submitter

iProX dataset URI