PXD040550

PXD040550 is an original dataset announced via ProteomeXchange.

Title	S. aureus exoproteome in the context of genetic competence development
Description	To perform natural transformation, one of the three main Horizontal Gene Transfer mechanisms, bacteria need to enter a physiological differentiated state called genetic competence. Interestingly, new bacteria displaying such aptitude are often discovered, and one of the latest is the human pathogen Staphylococcus aureus. Here, we developed a new protocol, based on planktonic cells cultures, leading to a large percentage of the population activating competence development and a significant improvement of S. aureus natural transformation efficiencies. Taking advantage of these new conditions, we performed transcriptomics analyses to characterize each central competence regulators regulon. SigH and ComK1 were both found essential for activating natural transformation genes but also important for activation or repression of peripheral functions. Even though ComK2 was not found important for the control of transformation genes, its regulon showed an important overlap with that of SigH and ComK1. Finally, we showed how microaerobic conditions, sensed by the SrrAB two-component system, was key to activate competence in S. aureus.
HostingRepository	PRIDE
AnnounceDate	2023-04-26
AnnouncementXML	Submission_2023-04-26_13:19:42.421.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040550
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	DavidCornu
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-02 05:26:19	ID requested
⏵ 1	2023-04-26 13:19:42	announced

10.6019/PXD040550;

submitter keyword: mass spectrometry,exoproteome

DavidCornu
contact affiliation	Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.
contact email	david.cornu@i2bc.paris-saclay.fr
lab head
DavidCornu
contact affiliation	SICaPS, Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay 91198 Gif-sur-Yvette Cedex, France
contact email	david.cornu@i2bc.paris-saclay.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040550

PRIDE project URI

• PRIDE
  1. PXD040550
     1. Label: PRIDE project
     2. Name: S. aureus exoproteome in the context of genetic competence development