PXD040547

PXD040547 is an original dataset announced via ProteomeXchange.

Title	Tissue-specific O-GlcNAcylation profiling reveals enrichment of ribosomal substrates in Drosophila mushroom body critical for associative learning
Description	O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome with spatiotemporal precision in response to various stimuli. Its dysregulation is associated with many neurological disorders that impair cognitive function, and yet identification of phenotype-relevant protein substrates in specific brain regions remains unfeasible. By combining O-GlcNAc binding activity of Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific substrates enrichment. We mapped the O-GlcNAcylated proteome in different brain regions of Drosophila and revealed that components in translational machinery including many ribosomal subunits are heavily O-GlcNAcylated in mushroom body (MB), the major brain region for associative learning. Hypo-O-GlcNAcylation induced by ectopic expression of CpOGA in MB reduces ribosomal activity, leading to olfactory learning deficit. Our study provides a useful resource for dissecting tissue-specific functions of O-GlcNAcylation in Drosophila and suggests that new protein synthesis is important for cognitive function.
HostingRepository	PRIDE
AnnounceDate	2024-04-10
AnnouncementXML	Submission_2024-04-10_07:41:12.745.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	haibin yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-02 03:22:00	ID requested
⏵ 1	2024-04-10 07:41:13	announced

10.7554/ELIFE.91269;

submitter keyword: Human, LC-MS, HEK293T cell, O-GlcNAc

Kai Yuan
contact affiliation	1.Hunan Key Laboratory of Molecular Precision Medicine, Department of Oncology, Xiangya Hospital & Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China
contact email	yuankai@csu.edu.cn
lab head
haibin yu
contact affiliation	Xiangya Hospital & Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China
contact email	13625484480@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD040547

PRIDE project URI

• PRIDE
  1. PXD040547
     1. Label: PRIDE project
     2. Name: Tissue-specific O-GlcNAcylation profiling reveals enrichment of ribosomal substrates in Drosophila mushroom body critical for associative learning