PXD040540
PXD040540 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deep Coverage of Arginine Phosphorylation Enriched in Metabolism Pathways of Mycolicibacterium smegmatis |
Description | Protein phosphorylation is one of the major posttranslational modifications that regulates protein expression and activity in bacteria. Although serine, threonine and tyrosine phosphorylation have been well-established, arginine phosphorylation has gained attention in recent decades due to its global importance in processes such as protein degradation, competence, stringent response, and other stress response. Mycolicibacterium smegmatis, a nonpathogenic surrogate of Mycobacterium tuberculosis, is a predominate model for studying the basic biology of mycobacterial pathogenesis. A recent study confirmed the presence of arginine phosphorylation in M. smegmatis and identified six arginine phosphorylated proteins based on shotgun proteomics analysis. Here, to provide a more comprehensive understanding, we performed a global, in-depth analysis of the M. smegmatis arginine phosphoproteome using high-accuracy mass spectrometry in combination with an optimized titanium-immobilized metal ion affinity chromatography (Ti4+-IMAC) phosphopeptide enrichment strategy. |
HostingRepository | iProX |
AnnounceDate | 2023-09-21 |
AnnouncementXML | Submission_2023-09-21_19:17:30.234.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiahui Shi |
SpeciesList | scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196; |
ModificationList | phosphorylated residue; phosphorylated residue with neutral loss of phosphate |
Instrument | Orbitrap Fusion; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-03-01 18:23:11 | ID requested | |
1 | 2023-03-01 18:23:18 | announced | |
⏵ 2 | 2023-09-21 19:17:30 | announced | 2023-09-22: Add Subproject |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mycolicibacterium smegmatis, arginine phosphorylation, proteomics |
Contact List
Yao Zhang | |
---|---|
contact affiliation | National Center for Protein Sciences (Beijing) |
contact email | zhangyaowsw@163.com |
lab head | |
Jiahui Shi | |
contact affiliation | National Center for Protein Sciences (Beijing) |
contact email | sjh1105211032@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |