PXD040518

PXD040518 is an original dataset announced via ProteomeXchange.

Title	A ParDE toxin-antitoxin system is responsible for maintenance of the Yersinia virulence plasmid, but not for type III secretion-associated growth inhibition
Description	Many Gram-negative pathogens utilize the type III secretion system (T3SS) to translocate virulence-promoting effector proteins into eukaryotic host cells. Activity of this system results in severe reduction of bacterial growth and division, summarized as secretion-associated growth inhibition (SAGI). In Y. enterocolitica, the T3SS and related proteins are encoded on a virulence plasmid. We identified a ParDE-like toxin-antitoxin system on this virulence plasmid in genetic proximity to yopE, encoding a T3SS effector. Effectors are strongly upregulated upon activation of the T3SS, indicating a potential role of the ParDE system in the SAGI or maintenance of the virulence plasmid. Expression of the toxin ParE in trans resulted in reduced growth and elongated bacteria, highly reminiscent of the SAGI. Nevertheless, activity of ParDE is not causal for the SAGI. T3SS activation did not influence ParDE activity; conversely, ParDE had no impact on T3SS assembly or activity itself. However, we found that ParDE ensures presence of the T3SS across bacterial populations by reducing loss of the virulence plasmid, especially under conditions relevant for infection. Despite this effect, a subset of bacteria lost the virulence plasmid and regained the ability to divide under secreting conditions, facilitating the possible emergence of T3SS-negative bacteria in late acute and persistent infections.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:47:29.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Yersinia enterocolitica subsp. enterocolitica NBRC 105693; NCBI TaxID: 1218100;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-01 01:54:56	ID requested
1	2023-06-09 02:17:57	announced
2	2023-11-14 09:00:40	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:47:30	announced	2024-10-22: Updated project metadata.

10.3389/fcimb.2023.1166077;

Schott S, Scheuer R, Ermoli F, Glatter T, Evguenieva-Hackenberg E, Diepold A, virulence plasmid but not for type III secretion-associated growth inhibition. Front Cell Infect Microbiol, 13():1166077(2023) [pubmed]

submitter keyword: Yersinia enterocolitica, T3SS

Timo Glatter
contact affiliation	Max Planck Insitut for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD040518

PRIDE project URI

• PRIDE
  1. PXD040518
     1. Label: PRIDE project
     2. Name: A ParDE toxin-antitoxin system is responsible for maintenance of the Yersinia virulence plasmid, but not for type III secretion-associated growth inhibition