PXD040508

PXD040508 is an original dataset announced via ProteomeXchange.

Title	Discovery of Itaconate-Mediated Lysine Acylation
Description	Itaconate is an important anti-microbial and immunoregulatory metabolite involved in host-pathogen interactions. A key mechanistic action of itaconate is through the covalent modification of cysteine residues via Michael addition, resulting in “itaconation”. However, it is unclear whether itaconate has other regulatory mechanisms. In this work, we discovered a novel type of post-translational modification by promiscuous antibody enrichment and data analysis with the open-search strategy, and further confirmed it as the lysine “itaconylation”. We showed that itaconylation undergo significant up-regulation upon lipopolysaccharides (LPS) stimulation in RAW264.7 macrophages. Quantitative proteomics identified itaconylation sites in several functional proteins, including glycolytic enzymes and histones, some of which were confirmed by synthetic peptide standards.
HostingRepository	iProX
AnnounceDate	2023-02-28
AnnouncementXML	Submission_2023-06-07_00:30:10.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongyang Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-28 18:32:51	ID requested
⏵ 1	2023-06-07 00:30:11	announced

Liu D, Xiao W, Li H, Zhang Y, Yuan S, Li C, Dong S, Wang C, Discovery of Itaconate-Mediated Lysine Acylation. J Am Chem Soc, 145(23):12673-12681(2023) [pubmed]

submitter keyword: itaconylation, itaconate, post-translational modification

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Dongyang Liu
contact affiliation	Peking University
contact email	sobeit@pku.edu.cn
dataset submitter

iProX dataset URI