PXD040501

PXD040501 is an original dataset announced via ProteomeXchange.

Title	LeishIF3d is a non-canonical cap-binding protein specific for Leishmania
Description	Translation of most cellular mRNAs in eukaryotes proceeds through a cap-dependent pathway, whereby the cap-binding complex, eIF4F, anchors the pre-initiation complex at the 5’ end of mRNAs driving translation initiation. The genome of Leishmania encodes a large repertoire of cap-binding complexes that fulfill a variety of functions possibly involved in survival along the life cycle. However, most of these complexes function in the promastigote life form that resides in the sand fly vector and lose their activity in amastigotes, the mammalian life form. Here we examined the possibility that LeishIF3d drives translation in Leishmania using alternative pathways. We describe a non-canonical cap-binding activity of LeishIF3d and examine its potential role in driving translation. LeishIF3d is required for translation, as reducing its expression by a homozygous deletion reduces the translation activity of the LeishIF3d(+/-) mutant cells. Proteomic analysis of the mutant cells highlights the reduced expression of flagellar and cytoskeletal proteins, as reflected in the morphological changes observed in the mutant cells. Targeted mutations in two predicted alpha helices diminish the cap-binding activity of LeishIF3d. Overall, LeishIF3d could serve as a driving force for alternative translation pathways, although it does not seem to offer an alternative pathway for translation in amastigotes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:26.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keren Bendalak
SpeciesList	scientific name: Leishmania mexicana mexicana; NCBI TaxID: 44270;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-28 07:28:40	ID requested
1	2023-07-20 11:41:29	announced
⏵ 2	2023-11-14 09:03:29	announced	2023-11-14: Updated project metadata.

Bose P, Baron N, Pullaiahgari D, Ben-Zvi A, Shapira M, . Front Mol Biosci, 10():1191934(2023) [pubmed]

submitter keyword: Leishmania, IF3d

Michal Shapira
contact affiliation	1Department of Life Sciences, Ben-Gurion University of the Negev Beer Sheva, 84105 Israel
contact email	shapiram@bgu.ac.il
lab head
Keren Bendalak
contact affiliation	Technion
contact email	kerenb@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD040501

PRIDE project URI

• PRIDE
  1. PXD040501
     1. Label: PRIDE project
     2. Name: LeishIF3d is a non-canonical cap-binding protein specific for Leishmania