PXD040487

PXD040487 is an original dataset announced via ProteomeXchange.

Title	The role of Ptpn11E76K in MSC fate determination
Description	MSCs expressing Ptpn11+/+ or Ptpn11E76K/+ were lysed for proteomic analysis. In brief, 0.5 μg of peptide mixture resolved in buffer A (0.1% formic acid) was loaded onto a 2‒cm self‒packed trap column using buffer A and separated on a 150 μm‒inner‒diameter column with a length of 15 cm over a 78‒min gradient at a flow rate of 600 nl/min. An Orbitrap Fusion mass spectrometer (Thermo Fisher) was operated in positive‒ion mode at an ion transfer tube temperature of 320°C. The positive‒ion spray voltage was 2.0 kV. The Orbitrap Fusion Lumos was set to the OT–IT mode. For a full mass spectrometry survey scan, the target value was 5×105, and the scan ranged from 300 to 1400 m/z at a resolution of 120000 and a maximum injection time of 50 ms. For the MS2 scan, a duty cycle of 3 s was set with the top‒speed mode. Only spectra with a charge state of 2–6 were selected for fragmentation by higher‒energy collision dissociation with a normalized collision energy of 35%. The MS2 spectra were acquired in the ion trap in rapid mode with an AGC target of 7,000 and a maximum injection time of 35 ms, and the dynamic exclusion was set to 18 s.
HostingRepository	iProX
AnnounceDate	2023-02-28
AnnouncementXML	Submission_2023-02-27_23:49:26.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kan Chen
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 23:48:49	ID requested
⏵ 1	2023-02-27 23:49:27	announced

Dataset with its publication pending

submitter keyword: MSC, PTPN11

Kan Chen
contact affiliation	Anhui Medical University
contact email	chenkan@ahmu.edu.cn
lab head
Kan Chen
contact affiliation	Anhui Medical University
contact email	chenkan@ahmu.edu.cn
dataset submitter

iProX dataset URI