PXD040487
PXD040487 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The role of Ptpn11E76K in MSC fate determination |
Description | MSCs expressing Ptpn11+/+ or Ptpn11E76K/+ were lysed for proteomic analysis. In brief, 0.5 μg of peptide mixture resolved in buffer A (0.1% formic acid) was loaded onto a 2‒cm self‒packed trap column using buffer A and separated on a 150 μm‒inner‒diameter column with a length of 15 cm over a 78‒min gradient at a flow rate of 600 nl/min. An Orbitrap Fusion mass spectrometer (Thermo Fisher) was operated in positive‒ion mode at an ion transfer tube temperature of 320°C. The positive‒ion spray voltage was 2.0 kV. The Orbitrap Fusion Lumos was set to the OT–IT mode. For a full mass spectrometry survey scan, the target value was 5×105, and the scan ranged from 300 to 1400 m/z at a resolution of 120000 and a maximum injection time of 50 ms. For the MS2 scan, a duty cycle of 3 s was set with the top‒speed mode. Only spectra with a charge state of 2–6 were selected for fragmentation by higher‒energy collision dissociation with a normalized collision energy of 35%. The MS2 spectra were acquired in the ion trap in rapid mode with an AGC target of 7,000 and a maximum injection time of 35 ms, and the dynamic exclusion was set to 18 s. |
HostingRepository | iProX |
AnnounceDate | 2023-02-28 |
AnnouncementXML | Submission_2025-06-11_01:30:15.110.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kan Chen |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-02-27 23:48:49 | ID requested | |
1 | 2023-02-27 23:49:27 | announced | |
⏵ 2 | 2025-06-11 01:30:16 | announced | 2025-06-11: Add Publication. |
Publication List
Kan C, Tan Z, Liu L, Liu B, Zhan L, Zhu J, Li X, Lin K, Liu J, Liu Y, Yang F, Wong M, Wang S, Zheng H, Phase separation of SHP2E76K promotes malignant transformation of mesenchymal stem cells by activating mitochondrial complexes. JCI Insight, 9(8):(2024) [pubmed] |
Keyword List
submitter keyword: MSC, PTPN11 |
Contact List
Kan Chen | |
---|---|
contact affiliation | Anhui Medical University |
contact email | chenkan@ahmu.edu.cn |
lab head | |
Kan Chen | |
contact affiliation | Anhui Medical University |
contact email | chenkan@ahmu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |