PXD040478 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomics measurement of clonal endocrine therapy sensitive and resistant breast cancer populations |
Description | Around two thirds of breast tumors are characterized by the expression of estrogen receptor α and are therapeutically treated by blocking estrogen signaling. First line endocrine therapeutics are Tamoxifen which displaces estrogen form its receptor preventing receptor activation and aromatase inhibitors which prevent the formation of estrogen and thereby hormone-deprive the tumors. Therapy resistance remains a major clinical problem, especially for patients with late-stage diagnoses. Tumor heterogeneity may promote therapy resistance either through the selection of pre-existing rare clones or by providing a repertoire of cells that may develop resistance over time. In order to study endocrine therapy resistance on a clonal level, we have developed barcoded (allowing the tracking of single cells) endocrine therapy sensitive and resistant breast cancer cell lines. From these complex cell pools, multiple single cells characterized by a specific barcode were isolated and grown out. We then subjected the clones to phosphoproteomics measurement by Mass spectrometry. Based on their phosphorylation pattern, the kinase activity profiles of endocrine therapy resistant clones were determined to identify differentially activated kinases with implications in therapy resistance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-27 |
AnnouncementXML | Submission_2024-01-26_17:11:16.849.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lukas Beumers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-27 10:01:43 | ID requested | |
⏵ 1 | 2024-01-26 17:11:18 | announced | |
Publication List
Beumers L, Vlachavas EI, Borgoni S, Schwarzm, ü, ller L, Penso-Dolfin L, Michels BE, Sofyali E, Burmester S, Heiss D, Wilhelm H, Yarden Y, Helm D, Will R, Goncalves A, Wiemann S, Clonal heterogeneity in ER+ breast cancer reveals the proteasome and PKC as potential therapeutic targets. NPJ Breast Cancer, 9(1):97(2023) [pubmed] |
10.1038/s41523-023-00604-4; |
Keyword List
submitter keyword: Phosphoproteomics, clonal deconvolution,Breast cancer, therapy resistance |
Contact List
Prof. Dr. Stefan Wiemann |
contact affiliation | Molekulare Genomanalyse (B050) Deutsches Krebsforschungszentrum Im Neuenheimer Feld 580 69120 Heidelberg Germany |
contact email | s.wiemann@dkfz.de |
lab head | |
Lukas Beumers |
contact affiliation | DKFZ Heidelberg |
contact email | l.beumers@dkfz-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040478
- Label: PRIDE project
- Name: Phosphoproteomics measurement of clonal endocrine therapy sensitive and resistant breast cancer populations