PXD040470

PXD040470 is an original dataset announced via ProteomeXchange.

Title	Proteomics Analysis of Extracellular Vesicles from In Vitro Models of the Human Airway.
Description	Extracellular vesicle (EV)-mediated intercellular communication significantly influences pulmonary cell health and disease outcomes, yet in vitro methods to investigate these pathophysiological mechanisms are limited. We hypothesize that organotypic models of the airway can be leveraged to investigate EV-mediated intercellular signaling, focusing on EV proteomic content as a case study. in this study, two in vitro airway culture models were evaluated using mass spectrometry-based proteomics: a tri-culture model consisting of alveolar epithelial, fibroblast, and lung microvascular endothelial cells and a co-culture model consisting of alveolar epithelial and fibroblast cells. EVs isolated from the tri-culture model showed enrichment for EV proteins regulating RNA-to-protein translation. EVs isolated from the co-culture model were enriched with EV biogenesis and extracellular matrix signaling proteins. These model-specific differences suggest that different pulmonary cell types uniquely affect EV composition and the biological pathways influenced by the EV proteome in recipient cells. These findings inform future study designs surrounding EV-related pulmonary disease pathogenesis and therapeutics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:26:39.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angie Mordant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 08:42:11	ID requested
1	2024-01-26 08:11:43	announced
⏵ 2	2024-10-22 06:26:40	announced	2024-10-22: Updated project metadata.

Vitucci ECM, Carberry CK, Payton A, Herring LE, Mordant AL, McCullough SD, Rager JE, organotypic multi-cellular models. iScience, 26(11):108162(2023) [pubmed]

10.1016/j.isci.2023.108162;

submitter keyword: Proteome

LC-MS/MS

extracellular vesicles

In vitro models

intercellular communication

cell signaling

human airway

pulmonary disease

Julia Rager
contact affiliation	University of North Carolina, Chapel Hill. Department of Environmental Sciences and Engineering (ENVR; Institute for Environmental Health Solutions (IEHS; UNC Superfund Research Program (UNC-SRP; Center for Environmental Medicine and Lung Biology (CEMALB; Curriculum in Toxicology & Environmental Medicine (CiTEM)
contact email	jrager@unc.edu
lab head
Angie Mordant
contact affiliation	UNC Proteomics Core, Department of Pharmacology, University of North Carolina at Chapel Hill
contact email	angie_mordant@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD040470

PRIDE project URI

• PRIDE
  1. PXD040470
     1. Label: PRIDE project
     2. Name: Proteomics Analysis of Extracellular Vesicles from In Vitro Models of the Human Airway.