PXD040463

PXD040463 is an original dataset announced via ProteomeXchange.

Title	Long non-coding RNA CASC2 regulates osteoblasts matrix mineralization
Description	Long non-coding RNAs (lncRNAs) are master regulators of gene expression and have recently emerged as potential innovative therapeutic targets. The deregulation of lncRNA expression patterns has been associated with age-related and noncommunicable diseases, including osteoporosis and bone tumors. However, the specific role of lncRNAs in physiological or pathological conditions in the bone tissue still needs to be further clarified, for their exploitation as therapeutic tools. In the present study, we evaluate the potential of the lncRNA CASC2 as a regulator of osteogenic differentiation and mineralization. Results show that CASC2 expression is decreased during osteogenic differentiation of human bone marrow-derived Mesenchymal Stem/Stromal cells (MSCs). CASC2 knockdown using small interfering RNA (siCASC2) increases the expression of the late osteogenic marker Bone Sialoprotein (BSP), but does not impact ALP staining levels, or the expression of early osteogenic transcripts including RUNX2 and OPG. Although siCASC2 does not impact hMSC proliferation nor apoptosis, it promotes the mineralization of hMSC cultured under osteogenic-inducing conditions, as shown by the increase of calcium deposits. Mass spectrometry-based proteomic analysis revealed that 89 proteins are regulated by CASC2 at late osteogenic stages, including proteins associated with bone diseases or anthropometric and musculoskeletal traits. Specifically, the Cartilage Oligomeric Matrix Protein (COMP) is highly enhanced by CASC2 knockdown at late stages of osteogenic differentiation, at either transcriptional and protein level. Inhibition of COMP impairs osteoblasts mineralization as well as the expression of BSP levels. The results indicate that lncRNA CASC2 regulates late osteogenesis and mineralization in hMSC via COMP and BSP. In conclusion, this study suggests lncRNA CASC2 as a potential new therapeutic target in bone mineralization.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:48:07.863.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040463
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sara Moura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 06:17:53	ID requested
1	2023-06-14 02:40:48	announced
2	2023-11-14 06:41:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:48:08	announced	2024-10-22: Updated project metadata.

10.3389/FBIOE.2023.1155596;

10.6019/PXD040463;

submitter keyword: Extracellular Matrix, Mesenchymal stem/stromal cells, Bone,Long non-coding transcripts, Cell Differentiation

Maria Inês Almeida
contact affiliation	i3s - Instituto de Investigação e Inovação em Saúde; INEB - Instituto Engenharia Biomédica; ICBAS - Instituto de Ciências Biomédicas Abel Salazar.
contact email	ines.almeida@ineb.up.pt
lab head
Sara Moura
contact affiliation	i3s - Instituto de Investigação e Inovação em Saúde
contact email	sara.moura@i3s.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD040463

PRIDE project URI

• PRIDE
  1. PXD040463
     1. Label: PRIDE project
     2. Name: Long non-coding RNA CASC2 regulates osteoblasts matrix mineralization