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PXD040452

PXD040452 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProximity interactome of lymphatic VE-cadherin reveals mechanisms of junctional remodeling and reelin secretion.
DescriptionIn this study, we are the first to use the MiniTurboID generation of the proximity-dependent biotin identification (BioID) technology to illuminate how the lymphatic endothelial cell (LEC) VE-cadherin interactome changes during junctional remodeling in response to the lymphangiocrine factor adrenomedullin. Here, we created a UBC-driven lentiviral expression vector containing the coding sequence for a Ve-Cadehrin-V5-MiniTurboID fusion-protein. After transduction of LECs with Ve-Cadehrin-MiniTurboID lentivirus, we treated these cells with 100 nM adrenomedullin (AM) or vehicle and labeled proximal proteins with 50 µM for 2 hours to capture dynamic changes in the Ve-Cadehrin interactome during AM-induced junctional rearrangement. Biotin-labeled proximity interactors were isolated via streptavidin-affinity purification (AP) and identified using LC-MS/MS mass spectrometry. Our dataset consists of distinct biological replicates consisting of AP enriched proteins from whole cell lysates (WCL, n = 2) or plasma membrane (PM, n = 3) fractions. WCL fractions allow us to capture proximity networks involved in Ve-Cadehrin trafficking and recycling to the PM, while PM fractions allows us to examine membrane-specific changes in AM-induced adherens junctions assembly.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:57:42.929.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristine Mills
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmono N-acetylated residue; monohydroxylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-27 06:13:42ID requested
12024-09-05 06:50:03announced
22024-10-22 06:57:43announced2024-10-22: Updated project metadata.
Publication List
10.1038/S41467-024-51918-1;
Keyword List
submitter keyword: VE-cadherin, Exocyst, Lymphatic Endothelial Cells, Adherens Junctions, Reelin, BioID
Contact List
Kathleen Caron
contact affiliationDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill
contact emailkathleen_caron@med.unc.edu
lab head
Christine Mills
contact affiliationUniversity of North Carolina - Chapel Hill
contact emailallie.mills@unc.edu
dataset submitter
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Dataset FTP location
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