PXD040431

PXD040431 is an original dataset announced via ProteomeXchange.

Title	Cyberlindnera jadinii yeast as a functional protein source: Modulation of immunoregulatory pathways in the intestinal proteome of zebrafish (Danio rerio)
Description	Cyberlindnera jadinii yeast is a potential sustainable novel feed ingredient for aquaculture industries. Yeasts contain bio-active components and proteins such as beta-glucans, mannans, nucleic acids and proteins that can enhance fish immunity against the disease. In our study, we focused on the characterization of intestinal immunoregulatory pathways in zebrafish (Danio rerio) by quantifying the intestine proteins with isobaric tags for relative and absolute quantitation (iTRAQ) and 2D LC-MS/MS approach. Zebrafish were fed either a control diet (C) or a diet supplemented with autolyzed C. jadinii (ACJ). The KEGG pathways analysis revealed that compared with the control diet, the ACJ yeast diet induced an increased abundance of proteins related to arginine and proline metabolism, phagosome, C-lectin receptor signalling pathway, ribosome pathway and PPAR signalling pathway, which can modulate and enhance the innate response of zebrafish. Moreover, fish fed ACJ yeast diet also showed decreased abundance of proteins associated with inflammatory pathways including apoptosis, necroptosis and ferroptosis pathways. These findings support a mobilization of the innate immune response and a control of inflammatory-related pathways in the intestine of zebrafish. Our findings in the well annotated proteome of zebrafish enabled a detailed investigation of intestinal responses and provide insight into the health-beneficial effects of the yeast species C. jadinii relevant for aquaculture species.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:10:32.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Purushothaman Kathiresan
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 05:20:28	ID requested
⏵ 1	2024-05-24 01:10:33	announced

Purushothaman K, Crawford AD, Rocha SDC, G, ö, ksu AB, Lange BM, Mydland LT, Vij S, Qingsong L, Ø, verland M, Press CM, ). Heliyon, 10(5):e26547(2024) [pubmed]

10.1016/j.heliyon.2024.e26547;

submitter keyword: Non saccharomyces yeast, Innate immunity, Proteomics, KEGG pathways analysis

Prof. Charles McLean Press
contact affiliation	Faculty of Veterinary Medicine Department of Preclinical Sciences and Pathology (PREPAT), Norwegian University of Life Sciences (NMBU), ÅS, Norway
contact email	charles.press@nmbu.no
lab head
Purushothaman Kathiresan
contact affiliation	Norwegian University of Life Sciences
contact email	purushothaman.kathiresan@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040431
     1. Label: PRIDE project
     2. Name: Cyberlindnera jadinii yeast as a functional protein source: Modulation of immunoregulatory pathways in the intestinal proteome of zebrafish (Danio rerio)